Leendert W. Schwab, Wouter M. J. Kloosterman, Jakob Konieczny, Katja Loos^*

Journal of Renewable Materials, Vol.1, No.1, pp. 73-78, 2013, DOI:10.7569/JRM.2012.634102

Abstract The papain catalyzed enzymatic synthesis of amido amines catalyzed from aromatic diamines and N-carbobenzyloxy (Z) protected amino acids (Gly, L-Leu, L-Phe) is described. The amides precipitate (yield 19–47 % depending on the amino acid used) from the reaction mixture after one amide bond is formed thus preventing the formation of diamides in all cases. Papain retains its activity in buffers with a higher pH (9 and 12) observable by the amide bond formation between 1,3-phenylene diamine and Z-L-Gly and Z-L-Phe. Aliphatic diamines (1,4-butanediamine and 1,6-hexanediamine) were used as well but amide formation could not be observed in buffers of pH… More >

Yanfei Feng, Jiaming Lin, Longxing Niu, Peng Pan, Xueping Liu, Linling Huang, Ying Guo, Mingzhong Li^*

Journal of Renewable Materials, Vol.11, No.1, pp. 167-184, 2023, DOI:10.32604/jrm.2022.021339

Abstract The development of a method for the quantitative determination of the sericin content ratio (SCR) is urgently needed for silk refining and the purification of silk fibroin for biomedical applications. In this work, a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk. Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin, and several linear relationships of SCR associated with the content ratios of Ser/Ala, Asp/Ala, Lys/Ala, Asp/Gly and Ser/Gly were established by… More > Graphic Abstract

Talha Imtiaz Baig^1,*, Yaser Daanial Khan¹, Talha Mahboob Alam², Bharat Biswal³, Hanan Aljuaid⁴, Durdana Qaiser Gillani⁵

CMC-Computers, Materials & Continua, Vol.71, No.1, pp. 215-230, 2022, DOI:10.32604/cmc.2022.021849

Abstract Lysine Lipoylation is a protective and conserved Post Translational Modification (PTM) in proteomics research like prokaryotes and eukaryotes. It is connected with many biological processes and closely linked with many metabolic diseases. To develop a perfect and accurate classification model for identifying lipoylation sites at the protein level, the computational methods and several other factors play a key role in this purpose. Usually, most of the techniques and different traditional experimental models have a very high cost. They are time-consuming; so, it is required to construct a predictor model to extract lysine lipoylation sites. This study proposes a model that… More >

Ahmed S. Sakr¹, M. Y. Shams², Amena Mahmoud³, Mohammed Zidan^4,*

CMC-Computers, Materials & Continua, Vol.70, No.1, pp. 123-134, 2022, DOI:10.32604/cmc.2022.019455

Abstract In this new information era, the transfer of data and information has become a very important matter. Transferred data must be kept secured from unauthorized persons using cryptography. The science of cryptography depends not only on complex mathematical models but also on encryption keys. Amino acid encryption is a promising model for data security. In this paper, we propose an amino acid encryption model with two encryption keys. The first key is generated randomly using the genetic algorithm. The second key is called the protein key which is generated from converting DNA to a protein message. Then, the protein message… More >

Chenggang Liang^1,#, Chunyu Wei^1,#, Yan Wang^1,*, Wujuan Yu¹, Kai Liao¹, Taoxiong Shi¹, Dabing Xiang^2,3, Qijiao Chen¹

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 871-893, 2021, DOI:10.32604/phyton.2021.014242

Abstract Rice-Tartary buckwheat (RTB) is a special germplasm of Tartary buckwheat. In this study, the appraisal of taste quality between RTB and conventional Tartary buckwheat (CTB) was presented, and the metabolites in kernels at three typical grain filling stage (GFS) were investigated. Unlike CTB, RTB showed thin shell seeds without longitudinal furrows at maturity, which was easily artificially dehulled. Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance, viscosity, taste and summary were appraised. In total, 92 metabolites were identified in kernels using GM-MS metabolomics platform. The levels of most metabolites changed greatly… More >

Erkan Arslan^1,*, Ismail Koyuncu²

Oncologie, Vol.23, No.1, pp. 105-117, 2021, DOI:10.32604/Oncologie.2021.014764

Abstract Prostate cancer is the second most common cancer in men. Prostate-specific antigen (PSA) levels, commonly used in the diagnosis of prostate cancer, are increased in both malign and benign conditions, such as prostate hyperplasia (BPH) and prostatitis. Thus, more specific markers are urgently needed to discriminate between prostate cancer and benign diseases of the prostate. The purpose of this study is to examine both the intracellular and extracellular free amino acid profiles of metastatic prostate cancer cells (PC-3), normal prostate cells (PNT-1A), and metabolic changes (e.g., pH). In this study, cancer and normal cells were incubated in the appropriate medium.… More >

Feng Liu^1,2,3, Zilan Ma^1,2,3, Marcos A. Caraballo-Ortiz⁴, Hui Zhang⁵, Xu Su^1,2,3, Yuping Liu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239

Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic acids were identified… More >

MINGHAO LI¹, YOULI QIU², WENHUI ZHANG¹, RUIHAO SUN¹, MEIJIN DU¹, LUZE YANG³, YU LI^1,*

BIOCELL, Vol.44, No.2, pp. 247-255, 2020, DOI:10.32604/biocell.2020.08835

Abstract While phthalate acid esters (PAEs) cannot fluoresce alone, they can be detected by fluorescence spectroscopy after chelation with bovine serum albumin (BSA). In this study, the types of amino acid residues at the active site of PAEs chelated with BSA were determined using molecular docking technology. A modification scheme of BSA with higher detection sensitivity fluorescence spectroscopy for PAEs was proposed based on the docking results and constructed for a novel BSA structure with a higher detection sensitivity of fluorescence spectroscopy using a homologous modeling method. Density functional theory (DFT) was employed to explore the influence before and after BSA… More >

Sacristán M, AM Millanes, B Fontaniella, ME Legaz, C Vicente

Phyton-International Journal of Experimental Botany, Vol.77, pp. 253-262, 2008, DOI:10.32604/phyton.2008.77.253

Abstract Secreted arginases from Evernia prunastri and Xanthoria parietina thalli, exhibiting lectin activity, have been purified to homogeneity. Analyses of both amino acid and glycoside composition are reported. Purified proteins have been subjected to tryptic digestion and some amino acid sequences have been analyzed. An undecapeptide from Evernia arginase, which shows some degree of homology with the domain to which Mn²⁺ binds, a heptapeptide and an undecapeptide from Xanthoria arginase have been analyzed, and homologies with some arginases from other fungi and plants have been found. More >

Lara-Rivera AH¹, MA Ruiz-Lopez¹, R Rodriguez-Macias¹, C Soto-Velasco¹, PM Garcia-López¹, L Barrientos-Ramirez², JF Zamora-Natera¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 89-96, 2017, DOI:10.32604/phyton.2017.86.089

Abstract Given that the agronomic potential of Lupinus angustifolius is not yet known in Mexico, we evaluated the yield and chemical composition of six L. angustifolius varieties (Haags Blaue, Boregine, Borlu, Probor, Sonate, and Boruta) in Zapopan, Jalisco, Mexico. Studies were conducted during the Autumn-Winter seasons of 2012-2013. We identified the varieties with the highest and lowest protein concentrations and analyzed their respective amino acid profiles. The major protein constituents were determined by electrophoresis (SDS-PAGE). This experiment was conducted in an agricultural facility at the University of Guadalajara using a completely randomized block design and four replicates. The highest grain yield… More >