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  • Open Access


    A Novel Method for the Quantitative Detection of Sericin Content in Silk Fiber Based on the Ratio of Aspartate to Alanine

    Yanfei Feng, Jiaming Lin, Longxing Niu, Peng Pan, Xueping Liu, Linling Huang, Ying Guo, Mingzhong Li*

    Journal of Renewable Materials, Vol.11, No.1, pp. 167-184, 2023, DOI:10.32604/jrm.2022.021339

    Abstract The development of a method for the quantitative determination of the sericin content ratio (SCR) is urgently needed for silk refining and the purification of silk fibroin for biomedical applications. In this work, a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk. Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin, and several linear relationships of SCR associated with the content ratios of Ser/Ala, Asp/Ala, Lys/Ala, Asp/Gly and… More > Graphic Abstract

    A Novel Method for the Quantitative Detection of Sericin Content in Silk Fiber Based on the Ratio of Aspartate to Alanine

  • Open Access


    Grain yield, and chemical and protein composition of Lupinus angustifolius varieties grown in Mexico

    Lara-Rivera AH1, MA Ruiz-Lopez1, R Rodriguez-Macias1, C Soto-Velasco1, PM Garcia-López1, L Barrientos-Ramirez2, JF Zamora-Natera1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 89-96, 2017, DOI:10.32604/phyton.2017.86.089

    Abstract Given that the agronomic potential of Lupinus angustifolius is not yet known in Mexico, we evaluated the yield and chemical composition of six L. angustifolius varieties (Haags Blaue, Boregine, Borlu, Probor, Sonate, and Boruta) in Zapopan, Jalisco, Mexico. Studies were conducted during the Autumn-Winter seasons of 2012-2013. We identified the varieties with the highest and lowest protein concentrations and analyzed their respective amino acid profiles. The major protein constituents were determined by electrophoresis (SDS-PAGE). This experiment was conducted in an agricultural facility at the University of Guadalajara using a completely randomized block design and four replicates. The… More >

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