Maria Babar¹, Siddra Ijaz^1,*, Imran Ul Haq², Muhammad Sarwar Khan¹

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1289-1300, 2023, DOI:10.32604/phyton.2023.026469

Abstract We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of MB-CLsRG gene encoding protein… More >

Henry López-López¹, Nancy Ruiz-Lau², Rocío Meza-Gordillo¹, Víctor Manuel Ruiz-Valdiviezo¹, Joseph Galthier Robledo-Luchetti¹, Carlos Alberto Lecona-Guzmán¹, Juan José Villalobos-Maldonado¹, Luc Dendooven³, Joaquín Adolfo Montes-Molina^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1235-1255, 2023, DOI:10.32604/phyton.2023.025716

Abstract The objective of this work was to evaluate the effect of Beauveria bassiana (Bb 1205) on controlling Fusarium oxysporum f. sp. lycopersici (Fol 17108) in tomato plants in greenhouse conditions. Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymes β-1,3-glucanases and chitinases. Inoculation of Bb 1205 occurred at a concentration of 1 × 10⁸ conidia·mL⁻¹, which was administered onto the leaves, directly into the soil and via injection. Infection with Fol 17108 occurred with 1 × 10⁶ spores·mL⁻¹, which were added directly to the soil. Spectrophotometry was used for… More >

Hernández-Castillo¹ FD, RH Lira-Saldivar², L Cruz-Chávez¹, G Gallegos-Morales¹, ME Galindo-Cepeda¹, E Padrón-Corral¹, M Hernández-Suárez²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 241-252, 2008, DOI:10.32604/phyton.2008.77.241

Abstract Potato crop requires more fungicides than any other crop in Mexico to prevent and control several diseases. More than 21,3% of the total available fungicides are required for cropping potato. High quantities of synthetic pesticides are intended to control Phytophthora infestans and Rhizoctonia solani fungi. As a result, this produces severe health problems and ecosystem disturbances. There is then an urgent need for finding new options for sustainable management of potato crop diseases. Several experiments were conducted under laboratory, greenhouse, and field conditions, to: (1) analyze the antifungal effect of bacteria strains of the genus Bacillus, and their effect on… More >