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  • Open Access

    ARTICLE

    Chilling Injury, Physicochemical Properties, and Antioxidant Enzyme Activities of Red Pitahaya (Hylocereus polyrhizus) Fruits under Cold Storage Stress

    Kai Sheng1, Saichao Wei1, Jun Mei1,2,3,4,*, Jing Xie1,2,3,4,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 291-305, 2021, DOI:10.32604/phyton.2020.012985 - 20 November 2020

    Abstract Low-temperature storage is extensively used to optimize the postharvest life of various fresh fruits. However, red pitahaya (Hylocereus polyrhizus) fruits are sensitive to chilling injury (CI), which leads to the limitation of low-temperature storage. In this study, red pitahaya fruits were stored at 2, 4, 6, 8, and 10°C, respectively, for 27 days to determine the appropriate storage temperature. During the storage of red pitahaya fruits, storage at 8°C was more effective in suppressing decay and maintaining quality than other low temperatures. Low-temperature (2, 4, and 6°C) storage decreased weight loss (WL) and maintained higher content… More >

  • Open Access

    ARTICLE

    Effects of Barium Stress in Brassica juncea and Cakile maritima: The Indicator Role of Some Antioxidant Enzymes and Secondary Metabolites

    Houda Bouslimi1, Renata Ferreira2, Nesrine Dridi1, Pedro Brito3, Susete Martins-Dias4, Isabel Caçador5, Noomene Sleimi1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 145-158, 2021, DOI:10.32604/phyton.2020.011752 - 20 November 2020

    Abstract Soil contamination by toxic trace metal elements, like barium (Ba), may stimulate various undesirable changes in the metabolic activity of plants. The plant responses are fast and with, direct or indirect, generation of reactive oxygen species (ROS). To cope with the stress imposed by the ROS production, plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS, and their by-products, into stable nontoxic molecules. To assess the Ba stress response of two Brassicaceae species (Brassica juncea, a glycophyte, and Cakile maritime, a halophyte), plants were exposure to different Ba concentrations (0, 100, 200,… More >

  • Open Access

    ARTICLE

    FCY-302, a Novel Small Molecule, Induces Apoptosis in Leukemia and Myeloma Cells by Attenuating Key Antioxidant and Mitochondrial Enzymes

    Prasanna Rajagopalan*†, Abdulrahim Hakami*†, Mohammed Ragab*, Ashraf Elbessoumy*‡

    Oncology Research, Vol.27, No.8, pp. 957-964, 2019, DOI:10.3727/096504019X15555428221646

    Abstract Arylidene analogs are well proven for biological activities. FCY-302, a novel small molecule belonging to this class, was screened for its biological efficacy in leukemia and myeloma cells. FCY-302 selectively inhibited proliferation of cancer cells with GI50 values of 395.2 nM, 514.6 Nm, and 642.4 nM in HL-60, Jurkat, and RPMI-8226 cells, respectively. The compound also increased sub-G0 peak in the cancer cell cycle and favored apoptosis determined by annexin V assay. The compound decreased the antiapoptotic Bcl-2 levels and increased proapoptotic Bax proteins in leukemia and myeloma cell lines. FCY-302 attenuated the mitochondrial membrane-bound Na+ More >

  • Open Access

    ARTICLE

    The Allelopathic Effects of Sunflower and Wheat Root Exudates on Sinapis arvensis and Sinapis alba

    Bengu Turkyilmaz Unal1,*, Mustafa Bayram2

    Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 413-423, 2019, DOI:10.32604/phyton.2019.08244

    Abstract In this study, we aimed to investigate the allelopathic effects of sunflower and wheat root exudates on the common weeds such as wild mustard and white mustard in our region. The root exudates which were obtained by soaking 8 weeks old sunflower and wheat seedlings (20 or 40 seedlings) in 100 mL of distilled water for 3 days were applied to the leaves of wild mustard and white mustard. In order to compare the allelopathic effect, the recommended dose (1 g.da-1 ) and twice the recommended dose (2 g.da-1 ) of Gromstor (Tribenuron-methyl), a herbicide preferred… More >

  • Open Access

    ARTICLE

    Physiological, Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress

    Xiaoqin Zhang, Tao Tong, Bin Tian, Yunxia Fang, Jiangjie Pan, Junjun Zheng and Dawei Xue*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 253-260, 2019, DOI:10.32604/phyton.2019.06143

    Abstract Barley (Hordeum vulgare L.) is one of the most Aluminum (Al) sensitive cereal species. In this study, the physiological, biochemical, and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms. The results showed that superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were inhibited to different degrees following Al exposure. The MDA content also significantly increased with increasing Al concentrations. SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile, and the genomic template stability (GTS) decreased with increasing More >

  • Open Access

    ARTICLE

    Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194

    Qunqun Hao1,#, Bo Lyu2,#, Yuhan Tang1,#, Deya Wang1, Yuanyuan Li1, Qingliang Li1, Yuhai Wang1,* and Wenqiang Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 109-117, 2019, DOI:10.32604/phyton.2019.06734 - 10 May 2024

    Abstract A barley mutant, 194, was observed to exhibit a leaf spot phenotype over the whole course of its growing period. In this study, the phenotype and antioxidant competence were studied in the lesion mimic mutant 194. Plant height was slightly higher in mutant 194 than in the wild type (WT). In addition, leaf spot per plant in mutant 194 was significantly higher than in WT. Antioxidant competence, as indicated by reactive oxygen species (ROS) accumulation, antioxidant enzyme activity, and the expression of antioxidant enzyme-encoding genes was also assessed in mutant 194. Compared to the WT, More >

  • Open Access

    ARTICLE

    Effect of mixed salt stress on malondialdehyde, proteins and antioxidant enzymes of Leymus chinensis in three leaf colors

    Zhou C1,2, CA Busso3, YG Yang2, Z Zhang4, ZW Wang1, YF Yang5, XG Han1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 205-213, 2017, DOI:10.32604/phyton.2017.86.205

    Abstract The mixed salt stress is common in nature. Salt stress always affects plant growth. Different plant species have different adaptive capacity to salty soils. Leymus chinensis is an herbaceous plant with different leaf colors. However, little research was conducted to explore the different tolerance mechanisms to salt stress among the three different leaf colour genotypes of Leymus chinensis (grey green, transitional color, yellow green). Pot experiments for Leymus chinensis in three leaf colors were conducted under mixed salt treatments in 2010. Malondialdehyde (MDA) and protein concentrations, and the activity of various antioxidant enzymes [i.e., superoxide dismutase (SOD), catalase… More >

  • Open Access

    ARTICLE

    Selenium invoked antioxidant defense system in Azolla caroliniana plant

    Hassan AMA, EM Mostafa

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 262-269, 2016, DOI:10.32604/phyton.2016.85.262

    Abstract Plants of Azolla caroliniana were treated with different selenium concentrations (0, 1, 2, 5, 7, 10 ppm) for seven days. Selenium (Se) content in Azolla plants increased significantly with increasing Se concentrations in the culture media up to 5 ppm. This indicated that Azolla plants were a good accumulator for Se. Selenium accumulation determined changes in Azolla biomass, doubling time and relative growth rates. Treatment of Azolla plants with low concentrations of Se (1 ppm) resulted in a significant increase in biomass. This was accompanied by a reduction in hydrogen peroxide and malondialdehyde (MDA) contents; the decrease percentages… More >

  • Open Access

    ARTICLE

    Comparison of isozyme transformation in maize as a result of insertion of the chitinase gene

    Yan PM1, HF Zhang1, Q Wang1, XY Yan1, Y Sun2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 117-121, 2010, DOI:10.32604/phyton.2010.79.117

    Abstract Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud… More >

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