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  • Open Access

    ARTICLE

    Selenium invoked antioxidant defense system in Azolla caroliniana plant

    Hassan AMA, EM Mostafa

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 262-269, 2016, DOI:10.32604/phyton.2016.85.262

    Abstract Plants of Azolla caroliniana were treated with different selenium concentrations (0, 1, 2, 5, 7, 10 ppm) for seven days. Selenium (Se) content in Azolla plants increased significantly with increasing Se concentrations in the culture media up to 5 ppm. This indicated that Azolla plants were a good accumulator for Se. Selenium accumulation determined changes in Azolla biomass, doubling time and relative growth rates. Treatment of Azolla plants with low concentrations of Se (1 ppm) resulted in a significant increase in biomass. This was accompanied by a reduction in hydrogen peroxide and malondialdehyde (MDA) contents; the decrease percentages were 78%… More >

  • Open Access

    ARTICLE

    Comparison of isozyme transformation in maize as a result of insertion of the chitinase gene

    Yan PM1, HF Zhang1, Q Wang1, XY Yan1, Y Sun2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 117-121, 2010, DOI:10.32604/phyton.2010.79.117

    Abstract Isozymes of peroxidase (POD), catalase (CAT), esterase (EST) and superoxide dismutase (SOD) were analyzed on transgenic maize (with external chitinase gene) and its parent by vertical polyacrylamide gel electrophoresis (PAGE). This study was made using shoots at the fourth leaf stage. Results showed that: POD and EST were detected in 6 bands. POD-2 and POD-3 were present at the bud and seedling stages. POD-1, POD-4, POD-5 and POD-6 were only present at the seedling stage. POD-6 expressed stronger in the transgenic maize with chitinase than in its parent. EST-2 was present only at the bud stage, and its expression in… More >

  • Open Access

    ARTICLE

    Effect of mixed salt stress on malondialdehyde, proteins and antioxidant enzymes of Leymus chinensis in three leaf colors

    Zhou C1,2, CA Busso3, YG Yang2, Z Zhang4, ZW Wang1, YF Yang5, XG Han1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 205-213, 2017, DOI:10.32604/phyton.2017.86.205

    Abstract The mixed salt stress is common in nature. Salt stress always affects plant growth. Different plant species have different adaptive capacity to salty soils. Leymus chinensis is an herbaceous plant with different leaf colors. However, little research was conducted to explore the different tolerance mechanisms to salt stress among the three different leaf colour genotypes of Leymus chinensis (grey green, transitional color, yellow green). Pot experiments for Leymus chinensis in three leaf colors were conducted under mixed salt treatments in 2010. Malondialdehyde (MDA) and protein concentrations, and the activity of various antioxidant enzymes [i.e., superoxide dismutase (SOD), catalase (CAT), ascorbate… More >

  • Open Access

    ARTICLE

    Physiological, Biochemical and Molecular Responses of Barley Seedlings to Aluminum Stress

    Xiaoqin Zhang, Tao Tong, Bin Tian, Yunxia Fang, Jiangjie Pan, Junjun Zheng and Dawei Xue*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 253-260, 2019, DOI:10.32604/phyton.2019.06143

    Abstract Barley (Hordeum vulgare L.) is one of the most Aluminum (Al) sensitive cereal species. In this study, the physiological, biochemical, and molecular response of barley seedlings to Al treatment was examined to gain insight into Al response and tolerance mechanisms. The results showed that superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were inhibited to different degrees following Al exposure. The MDA content also significantly increased with increasing Al concentrations. SRAP results indicated significant differences between Al treatments and controls in terms of SRAP profile, and the genomic template stability (GTS) decreased with increasing Al concentration and duration. These… More >

  • Open Access

    ARTICLE

    Deterioration of Antioxidant Competence in Barley Lesion Mimic Mutant 194

    Qunqun Hao1,#, Bo Lyu2,#, Yuhan Tang1,#, Deya Wang1, Yuanyuan Li1, Qingliang Li1, Yuhai Wang1,* and Wenqiang Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 109-117, 2019, DOI:10.32604/phyton.2019.06734

    Abstract A barley mutant, 194, was observed to exhibit a leaf spot phenotype over the whole course of its growing period. In this study, the phenotype and antioxidant competence were studied in the lesion mimic mutant 194. Plant height was slightly higher in mutant 194 than in the wild type (WT). In addition, leaf spot per plant in mutant 194 was significantly higher than in WT. Antioxidant competence, as indicated by reactive oxygen species (ROS) accumulation, antioxidant enzyme activity, and the expression of antioxidant enzyme-encoding genes was also assessed in mutant 194. Compared to the WT, mutant 194 displayed a relatively… More >

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