TATIANA CARLA TOMIOSSO, LAURECIR GOMES, BENEDICTO DE CAMPOS VIDAL, EDSON ROSA PIMENTEL
BIOCELL, Vol.29, No.1, pp. 47-54, 2005, DOI:10.32604/biocell.2005.29.047
Abstract The composition and organization of the extracellular matrix of ostrich articular cartilage was investigated, using samples from the proximal and distal surfaces of the tarsometatarsus. For morphological analysis, sections were stained with toluidine blue and analyzed by polarized light microscopy. For biochemical analysis, extracellular matrix components were extracted with 4 M guanidinium chloride, fractionated on DEAE-Sephacel and analyzed by SDS-PAGE. Glycosaminoglycans were analyzed by electrophoresis in agarose gels. Structural analysis showed that the fibrils were arranged in different directions, especially on the distal surface. The protein and glycosaminoglycan contents of this region were higher than in the other regions. SDS-PAGE… More >
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