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  • Open Access

    ARTICLE

    Brassinosteroid Biosynthetic Gene CmDWF4 Regulates Bud Outgrowth in Chrysanthemum morifolium

    Xianrong Fu, Aiping Song, Bo Peng, Song Li, Weixin Liu, Lingling Zhang, Jiafu Jiang, Sumei Chen, Fadi Chen*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1681-1694, 2023, DOI:10.32604/phyton.2023.027870

    Abstract Brassinosteroids (BRs), a class of steroid phytohormones, play a critical role in plant growth and development. The DWF4 gene encodes a cytochrome P450 enzyme (CYP90B1), which is considered a rate-limiting enzyme in BR biosynthesis. Here, we identified a homologous gene of DWF4 in chrysanthemum, CmDWF4. This gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point (pI) of 9.10. Overexpression of CmDWF4 in chrysanthemum was found to significantly increase growth rate, number, and length of lateral buds. Transcriptome analysis showed that multiple xyloglucan endotransglycosylase/hydrolase (XTH) family encoding genes associated with… More >

  • Open Access

    ARTICLE

    In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis

    Gloria Viviana Cerrillo-Rojas1, Mariana Tiscareño-Andrade1, Ana Erika Ochoa-Alfaro2, Eugenio Pérez-Molphe Balch1, Ruth Elena Soria-Guerra2, José Francisco Morales-Domínguez1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 715-726, 2020, DOI:10.32604/phyton.2020.09769

    Abstract Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and, they are suitable for reclamation and remediation of saline soil. We develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis. Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators. The highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine (BA) and / or 2 g/L activated carbon with 5.5 ±… More >

  • Open Access

    ARTICLE

    Identification of axillary buds of potato seedlings based on a vision system with fuzzy logic

    Martínez Corral L1, E Martínez-Rubin2, F F lores-García3, M Vázquez-Rueda3, J Frías-Ramírez2, MA Segura-Castruita2

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 79-84, 2011, DOI:10.32604/phyton.2011.80.079

    Abstract Potato (Solanum tuberosum L.) is a crop whose production yield at national level is very low compared with that in the most productive countries. This is because it is a partially automated crop with deficient and inadequate agronomic practices, low technification levels and great quantity of work wages required per hectare of cultivation. The necessity to generate technical and modern procedures that increase crop production, quality and yield has fostered development of projects leading to obtain seedlings free of pathogens with material of high genetic, physiological and sanitary quality. Utilization of a vision system for the computerized visual recognition of… More >

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