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    ARTICLE

    Epi-Brassinolide Positively Affects Chlorophyll Content and Dark-Reaction Enzymes of Maize Seedlings

    Yuanfen Gao, Tinglan Jiang, Yin Xiang, Xuewu He, Zhen Zhang, Shengjuan Wen, Junjie Zhang*

    Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1465-1476, 2021, DOI:10.32604/phyton.2021.014811

    Abstract Brassinosteroids participate in many physiological processes in plants; however, their regulatory roles on the activities of the enzymes involved in dark phase of photosynthesis remains elusive. In this study, detached leaves and protoplasts of maize seedlings were treated with epi-brassinolide (EBR) and brassinazole followed by the determination of the contents of chlorophyll (a+b) and soluble sugars, and the activity of dark reaction enzymes and the expression of the relevant genes. The results showed that chlorophyll (a+b) content increased by 7.4% under 0.1 μM EBR treatment for 48 h; furthermore, chlorophyll (a+b) content increased by 34% in detached leaves that were… More >

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