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  • Open Access

    ARTICLE

    Meta-Analysis of Flowering-Related Traits and Mining of Candidate Genes in Maize

    Jianyu Lu1, Qingping Jiang1, Tongyu Liu1, Yangyang Zhou1, Mengtong Liu1, Jing Qu2, Yiyong Ma2,*, Shuyan Guan2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1199-1209, 2022, DOI:10.32604/phyton.2022.019298

    Abstract Maize flowering is an important agronomic character, which is controlled by quantitative trait loci (QTL). Over the years, a large number of flowering-related QTL have been found in maize and exist in public databases. However, combining these data, re-analyzing and mining candidate loci and fine mapping of flowering-related traits to reduce confidence intervals has become a hot issue in maize. In this study, the QTL of 6 important agronomic traits of maize flowering were collected from 15 published articles, including flowering period (DA), Days to tasseling (DTT), Days to silking (DS), Days to pollen shedding (DTP), anthesis-silking interval (ASI) and… More >

  • Open Access

    ARTICLE

    Identification of Single Nucleotide Polymorphisms and Analysis of Linkage Disequilibrium in Different Bamboo Species Using the Candidate Gene Approach

    Xihua Liu1, Min Luo1, Xiaofei Chen1, Changjun Ding2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1697-1709, 2021, DOI:10.32604/phyton.2021.015295

    Abstract Bamboos are one of the most beautiful and useful plants on Earth. The genetic background and population structure of bamboos are well known, which helps accelerate the process of artificial domestication of bamboo. Partial sequences of six genes involved in nitrogen use efficiency in 32 different bamboo species were analyzed for occurrence of single nucleotide polymorphisms (SNPs). The nucleotide diversity θw and total nucleotide polymorphisms πT of the sequenced DNA regions was 0.05137 and 0.03332, respectively. Both πnonsyn /πsyn and Ka/Ks values were <1. The nucleotide sequences of these six genes were inferred to be relatively conserved, and the haplotype… More >

  • Open Access

    ARTICLE

    Characterization and Candidate Gene Analysis of the Yellow-Green Leaf Mutant ygl16 in Rice (Oryza sativa L.)

    Linjun Cai1,#, Junhua Liu2,#, Han Yun1, Dan Du1, Xiaolong Zhong1, Zhenlin Yang1, Xianchun Sang1, Changwei Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1103-1117, 2021, DOI:10.32604/phyton.2021.015532

    Abstract Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis, photomorphogenesis, hormone physiology and disease resistance. In this study, the genetically stable yellow-green leaf mutant ygl16 was identified from mutated “Xinong 1B”. Compared with the wild type, the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly. The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts, and the grana and matrix lamellae were blurred and loose in varied degrees, and the chloroplast structure was disordered, while the osmiophilic corpuscles increased. The results of the genetic analysis… More >

  • Open Access

    ARTICLE

    Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from Gossypium hirsutum × Gossypium barbadense via Bulked Segregant RNA-Sequencing

    Qi Zhang1,#, Pengtao Li2,#, Aiying Liu1, Shaoqi Li1, Quanwei Lu2, Qun Ge1, Junwen Li1, Wankui Gong1, Xiaoying Deng1, Haihong Shang1,3, Yuzhen Shi1,*, Youlu Yuan1,3,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 837-858, 2021, DOI:10.32604/phyton.2021.014437

    Abstract Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC5F3:5 CSSLs (chromosome segment substitution lines) from Gossypium hirsutum CCRI36 × G. barbadense Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC7F2 with its recurrent parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed and sequenced, including two parents… More >

  • Open Access

    ARTICLE

    Fine Mapping and Candidate Gene Prediction of the Quantitative Trait Locus qPL8 for Panicle Length in Rice

    Lin Zhang1,2,*, Yiting Zou1, Zhong Bian1, Dong Xie1, Hadi Yeilaghi1, Xiaolei Fan1,2, Dongsheng Zhao1,2, Qiaoquan Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 789-802, 2021, DOI:10.32604/phyton.2021.014880

    Abstract Rice panicle is the sink organ where assimilation product accumulates, and its morphology determines the rice yield. Panicle length has been suggested as a yield-related trait, but the genetic factor for its control is still limited. In this study, we carried out fine-mapping of qPL8, a QTL identified for panicle length in our previous work. Near isogenic line (NIL) with qPL8 exhibited elongated panicle without obvious effect on other panicle elements. With five key recombinants from NIL population, the locus was finally narrowed down to a 278-kb region, where 44 genes are annotated. By comparing the genomic sequence of two… More >

  • Open Access

    ARTICLE

    Newly identified genetic variant rs2294693 in UNC5CL gene is associated with decreased risk of esophageal carcinoma in the J&K Population–India

    RUCHI SHAH1,*, SONALI VERMA1, AMRITA BHAT1, GH RASOOL BHAT1, VARUN SHARMA1, INDU SHARMA1, HEMENDER SINGH1, SANDEEP KAUL2, EKTA RAI1, SWARKAR SHARMA1,*

    BIOCELL, Vol.45, No.3, pp. 665-670, 2021, DOI:10.32604/biocell.2021.09629

    Abstract Esophageal cancer is the second most common type of cancer after lung carcinoma in the state of Jammu and Kashmir (J&K). The understanding of genetics in Esophageal cancer development is poor in the state. Genome wide association studies (GWAS) has proved to be unsurpassed tool in identification of new loci associated with different cancers. GWAS in Chinese population has identified SNP rs2294693 present in UNC5CL (UNC-5 Family C-Terminal like) to be associated with non-cardia gastric cancer. We performed a case control association study and genotyped the SNP rs2294693 using Taqman allele discrimination assay in 566 individuals (166 esophageal cancer patients… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification of Candidate Genes Associated with β-glucan Traits in a Hulled and Hulless Barley (Hordeum vulgare L.) Population

    Longhua Zhou1,2,#, Ting He1,2,#, Guimei Guo1,2, Jing Li1,2, Ruiju Lu1,2, Yingbo Li1,2,*, Chenghong Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 987-997, 2020, DOI:10.32604/phyton.2020.011728

    Abstract Barley grain is a valuable source of β-glucan, which is an important component of dietary fiber with significant human health benefits. Although the genetic basis of β-glucan biosynthesis has been widely studied, a genome-wide association study (GWAS) is still required for a scan of the candidate genes related to the complex quantitative trait based on the high-quality barley reference genome. In this study, a GWAS was conducted using a population composed of 87 barley landraces (39 hulled and 48 hulless, β-glucan from 2.07% to 6.56%) with 191,098 nucleotide polymorphisms (SNPs) markers to cover the chromosomes with the highest density. The… More >

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