Christopher A. Lemmon1,2, Nathan J. Sniadecki3, Sami Alom Ruiz1,3, John L. Tan, Lewis H. Romer2,4,5, Christopher S. Chen3,4
Molecular & Cellular Biomechanics, Vol.2, No.1, pp. 1-16, 2005, DOI:10.3970/mcb.2005.002.001
Abstract The interplay of mechanical forces between the extracellular environment and the cytoskeleton drives development, repair, and senescence in many tissues. Quantitative definition of these forces is a vital step in understanding cellular mechanosensing. Microfabricated post array detectors (mPADs) provide direct measurements of cell-generated forces during cell adhesion to extracellular matrix. A new approach to mPAD post labeling, volumetric imaging, and an analysis of post bending mechanics determined that cells apply shear forces and not point moments at the matrix interface. In addition, these forces could be accurately resolved from post deflections by using images of post tops and bases. Image… More >
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