NÉSTOR CURVETTO11,2, PABLO MARINANGELI1,2, GABRIELA MOCKEL2
BIOCELL, Vol.30, No.3, pp. 497-500, 2006, DOI:10.32604/biocell.2006.30.497
Abstract The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2 ) into the nutrient media as chemical sterilizer. A series of H2O2 concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits… More >
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