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  • Open Access


    Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on Agrobacterium Growth

    Abraham Lamboro1,3,*, Songnan Yang1, Xueying Li1, Dan Yao2, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2489-2501, 2023, DOI:10.32604/phyton.2023.029492

    Abstract The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut cultivars. The culture media contained 4 mg/L 6-benzylaminopurine (BAP) as the main growth regulator. Various concentrations (100–600 mg/L) of cefotaxime, carbenicillin, and timentin were applied in the culture media. In all the tested media, there were no significant differences in the shoot induction as compared to the control. However, little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation media. Under shoot elongation medium, shoots turned brownish and partly died at higher concentrations where shooting rates… More >

  • Open Access


    Combination of 6-Benzylaminopurine and Thidiazuron Promotes Highly Efficient Shoot Regeneration from Cotyledonary Node of Mature Peanut (Arachis hypogaea L.) Cultivars

    Abraham Lamboro1,*, Xiao Han1, Songnan Yang1, Xueying Li1, Dan Yao2, Baixing Song1, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2619-2631, 2022, DOI:10.32604/phyton.2022.021404

    Abstract Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied to evaluate shoot regeneration capacity whereas α-naphthalene acetic acid (NAA) combination with 6-benzylaminopurine (BAP), and 2, 4-dichlorophenoxyacetic acid (2, 4-D) with 6-benzylaminopurine were tested to optimize root induction for two peanut cultivars. The result showed combination (BAP with TDZ) was found to be effective in promoting shoot. The highest shoot regeneration frequency (93%) was obtained on a medium supplemented with 4 mg/L BAP and 0.5 mg/L TDZ while an average… More >

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