Yajun Li1,#, Yumeng Cui1,#, Bingjie Wang1, Yue Li1, Mengmeng Zhang2, Silan Dai1, He Huang1,*
Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659
Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L−1 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L−1 α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration media were MS +… More >
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