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    ARTICLE

    Evaluation of a multiplex PCR method to detect enteroaggregative Escherichia coli

    M.E. RÜTTLER, C.S. YANZÓN, M.J. CUITIÑO, N.F. RENNA, M.A. PIZARRO, A.M. ORTIZ.

    BIOCELL, Vol.30, No.2, pp. 301-308, 2006, DOI:10.32604/biocell.2006.30.301

    Abstract Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator (aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 (astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a… More >

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