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    ARTICLE

    RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct

    PABLO A. VALDECANTOS, MARTÍN E. ARGAÑARAZ, CARLOS M. ABATE, DORA C. MICELI

    BIOCELL, Vol.28, No.3, pp. 287-297, 2004, DOI:10.32604/biocell.2004.28.287

    Abstract As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this… More >

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