WANG JIANMIN, SONG RUIHUA, CHEN LEI, YIN LIANGJUN, CHEN BO, SUN JING, GOU YUANBING, ZHAO LING, CHEN LIN
BIOCELL, Vol.30, No.2, pp. 269-278, 2006, DOI:10.32604/biocell.2006.30.269
Abstract Objective: To investigate the functions of Fibroblast Growth Factor Receptor-2 (FGFR2) at different stages of cell differentiation. The engineered murine embryonic stem (ES) cells with conditional knockout of FGFR2 were developed depending on Cre-loxP. Methods: Cre-loxP system was used in a conditional targeting vector. The competent AM-1 bacteria, which expressed Cre-recombinase, was used to confirm the Cre-mediated deletion of the floxed exons 7 and 8 of FGFR2. The targeting vector was electroporated into the ES cells, and the transfected ES cells were screened with G418 and Ganciclovir. Finally, the ES clones with correct targeting events were identified by Southern Blot… More >
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