Yinjie Wang, Yongxia Zhang, Qingquan Liu, Liangqin Liu, Suzhen Huang, Haiyan Yuan*
Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 277-290, 2021, DOI:10.32604/phyton.2020.011545
Abstract Quantitative real-time PCR (qPCR) is an effective and widely used
method to analyze expression patterns of target genes. Selection of stable reference genes is a prerequisite for accurate normalization of target gene expression
by qRT-PCR. In Iris germanica L., no studies have yet been published regarding
the evaluation of potential reference genes. In this study, nine candidate reference
genes were assessed at different flower developmental stages and in different tissues by four different algorithms (GeNorm, NormFinder, BestKeeper, and RefFinder). The results revealed that ACT11 (Actin 11) and EF1α (Elongation factor
1 alpha) were the most stable reference genes in different… More >
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