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  • Open Access

    ARTICLE

    In vitro antifungal/fungistatic activity of manganese phosphite against soybean soil-borne pathogens

    Carmona MA1, E Simonetti2,3, ME Ravotti1, MM Sc,iani4, AG Luque4, NA Formento5, FJ Sautua1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

    Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC50) ranged from… More >

  • Open Access

    ARTICLE

    Chitinase, chitosanase, and antifungal activities from thermophilic streptomycetes isolated from compost

    González-Franco AC1, L Robles-Hernández1, JL Strap2

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 14-27, 2017, DOI:10.32604/phyton.2017.86.014

    Abstract The Streptomyces genus comprises a large and diverse group of bacteria, many of which are commercially exploited for the production of antibiotics and lytic enzymes. The thermophilic species are less studied than the predominant mesophilic species. However, the first ones are a potential source of thermostable bioactive products and enzymes with novel properties. In this study, two selected thermophilic streptomycetes were identified and their chitinolytic activities were evaluated. The identification of these two isolates was performed by microscopic morphology, partial 16S rDNA sequences, and its phylogenetic analysis. To study the chitinolytic activities of these isolates, the effects of colloidal chitin… More >

  • Open Access

    ARTICLE

    Effect of sugars on the association between cowpea vicilin (7S storage proteins) and fungal cells

    T.L. Rose*, V.M. Gomes*, M. Da Cunha**, K.V.S. Fernandes***, J. Xavier-Filho***

    BIOCELL, Vol.27, No.2, pp. 173-179, 2003, DOI:10.32604/biocell.2003.27.173

    Abstract Vicilins (7S storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. In the present work vicilins from cowpea (Vigna unguiculata) seeds were added to the growth medium of Saccharomyces cerevisiae cells and Fusarium oxysporum conidia. Helix pomatia lectin, wheat germ agglutinin and Ulex europaeus lectin were used to identify differences in the binding of the vicilins to the surface of cells of S. cerevisiae and F. oxysporum treated with this protein. After… More >

  • Open Access

    ARTICLE

    Antifungal activity of various essential oils against Saccharomyces cerevisiae depends on disruption of cell membrane integrity

    HATİCE BÜŞRA KONUK, BENGÜ ERGÜDEN*

    BIOCELL, Vol.41, No.1, pp. 13-18, 2017, DOI:10.32604/biocell.2017.41.013

    Abstract Antifungal activity and mode of action of nine essential oils (EOs) against S. cerevisiae cells were examined. Antifungal effects of commercial lemon peel, orange peel, tea tree, turpentine, rosemary, peppermint, thyme, oregano and clove oils were determined through Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC) and inhibition zone measurements. The most effective oil was turpentine oil. Orange peel, thyme and oregano oils were also effective, according to MIC and MFC. Inhibition zone measurements, also revealed oregano, orange peel, thyme, turpentine and clove oils as most efficient ones. Later, membrane damage of yeast cells was studied by measuring the extracellular… More >

  • Open Access

    ARTICLE

    Antifungal Activity of Schinifoline Against Candida Albicans in Caenorhabditis Elegans

    Chengjie Shu1, Mengli Zhang1, Weiming Zhang1, Shihong Ma1, Guoqing Chen2 and Baojun Shi1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 317-324, 2019, DOI:10.32604/phyton.2019.07766

    Abstract Zanthoxylum schinifolium has been used as spices and traditional medicine in China for hundreds of years. A variety of active substances have been isolated from Zanthoxylum schinifolium using biological and chemical techniques. Among these substances, the effect of schinifoline has gradually attracted much attention. Candida albicans is one of the most common pathogens isolated from the gastrointestinal tract, vagina, and mouth in healthy individuals. In a healthy population, there are various mechanisms in host, such as the microbial flora, the epithelial barriers, and the innate immune system, that can control the presence of Candida albicans. However, when host immunity is… More >

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