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  • Open Access

    ARTICLE

    Chemical Composition and Antifungal Efficacy of Mentha rotundifolia Essential Oil against Fusarium oxysporum f. sp. albedinis in Date Palm: Valorisation of Plant Biomass for Natural Antifungal Agents

    Hafida Khelafi1, Wassima Lakhdari2, Mustapha Mounir Bouhenna3, Said Boudeffeur4, Hayet Meamiche1, Salah Neghmouche Nacer5,*, Meriam Laouar6

    Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 3975-3989, 2025, DOI:10.32604/phyton.2025.073210 - 29 December 2025

    Abstract Essential oils (EOs) derived from medicinal plants are gaining recognition as sustainable alternatives to synthetic fungicides in the management of plant pathogens. This study investigates the chemical composition, chromatographic profile, and antifungal of Mentha rotundifolia essential oil against Fusarium oxysporum f. sp. albedinis (Foa), the pathogen responsible for Bayoud disease in date palm. The oil was extracted through hydrodistillation and characterized using thin-layer chromatography (TLC) and gas chromatography–mass spectrometry (GC-MS), revealing multiple fractions corresponding to terpenoid constituents and 23 chemical constituents, predominantly oxygenated monoterpenes (68.51%), with piperitenone oxide as the major component (62.53%). The antifungal efficacy was evaluated… More >

  • Open Access

    ARTICLE

    Sustainable Egg Packaging Waste Biocomposites Derived from Recycled Wood Fibers and Fungal Filaments

    Ilze Irbe1,*, Laura Andze1, Inese Filipova1,2

    Journal of Renewable Materials, Vol.13, No.11, pp. 2139-2154, 2025, DOI:10.32604/jrm.2025.02025-0107 - 24 November 2025

    Abstract Growing environmental concerns and the need for sustainable alternatives to synthetic materials have led to increased interest in bio-based composites. This study investigates the development and characterization of sustainable egg packaging waste (EPW) biocomposites derived from recycled wood fibers and fungal mycelium filaments as a natural binder. Three formulations were prepared using EPW as the primary substrate, with and without the addition of hemp shives and sawdust as co-substrates. The composites were evaluated for granulometry, density, mechanical strength, hygroscopic behavior, thermal conductivity, and fire performance using cone calorimetry. Biocomposites, composed exclusively of egg packaging waste,… More >

  • Open Access

    CASE REPORT

    Primary renal aspergilloma: a challenge to endure—a case report

    Sarbjit Mohapatra1,*, Nandkumar Madhekar2, Sameer Ratkal3, Jaideep Ratkal1

    Canadian Journal of Urology, Vol.32, No.5, pp. 509-513, 2025, DOI:10.32604/cju.2025.063783 - 30 October 2025

    Abstract Background: Isolated renal aspergilloma is a rare medical entity posing difficult diagnostic and treatment challenges. Case Description: We document a case of Primary Renal Aspergilloma in a 55-year-old male with diabetes mellitus, hypertension, and chronic liver disease. The individual presented with features of obstructive uropathy due to fungal balls requiring retrograde endoscopic intervention and drainage. The cheesy material retrieved during the procedure was identified as an Aspergilloma on histopathology. Due to far and fewer reports in the medical literature, there have been no standard diagnostic & management strategies, which in turn has resulted in unacceptably high More >

  • Open Access

    ARTICLE

    Ultrasound-Assisted Hydrothermal Treatment in Combination with Chitosan for Fungal Control of Botrytis cinerea

    Surelys Ramos-Bell1, Edson Rayón-Díaz1, Juan A. Herrera-González2, Estefanía Martínez-Batista1, Rita M. Velázquez-Estrada1, Porfirio Gutiérrez–Martínez1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3145-3156, 2025, DOI:10.32604/phyton.2025.071543 - 29 October 2025

    Abstract A significant portion of losses in the fruit and vegetable sector are caused by the necrotrophic fungus Botrytis cinerea, due to its high prevalence in over 1000 crop species. Identifying a technology capable of exclusively inhibiting its growth and development remains challenging; therefore, various treatments have been proposed to act synergistically in preventing the spread of this pathogen. The objective of this study was to evaluate an ultrasound-assisted treatment combined with chitosan to control the development of B. cinerea. In vitro analyses showed that combining temperature, ultrasound, and chitosan inhibited the fungal radial growth by 80%, reduced sporulation, More >

  • Open Access

    ARTICLE

    Crude Extract of Ulva lactuca L., Spirulina platensis (Gomont) Geitler and Nostoc muscorum C. Agardh ex Bornet & Flahault for Mitigating Powdery Mildew and Improving Growth of Cucumber

    Ahmed Mahmoud Ismail1,*, Eman Said Elshewy2, Ayman Y. Ahmed3, Hossam M. Darrag4

    Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3023-3045, 2025, DOI:10.32604/phyton.2025.067444 - 29 October 2025

    Abstract Powdery mildew of cucumber (Cucumis sativus L.) is a destructive disease caused by Podosphaera xanthii (Castagne) U.Braun & Shishkoff. This study aimed to investigate the antifungal effect of extracts of Ulva lactuca, Spirulina platensis, and Nostoc muscorum against P. xanthii and to improve the physiological and morphological traits of cucumber under commercial greenhouse conditions. The chemical composition of the individual extracts from U. lactuca, S. platensis, and N. muscorum was analyzed utilizing High-performance Liquid Chromatography (HPLC) and Gas Chromatography/Mass spectrometry (GC/MS). Cucumber plants were sprayed twice with 5% of the crude extracts of U. lactuca, S. platensis, and N. muscorum and their mixture (U. lactuca, S. platensis, and N. muscorum).… More >

  • Open Access

    ARTICLE

    Genomic and Functional Characterization of Thermophilic Paenibacillus sp. VCA1: A Biocontrol Agent Isolated from El Chichón Volcano Crater Lake

    Nancy Abril Martínez-López1, Betsy Anaid Peña-Ocaña2, Rodolfo García-Contreras3, Toshinari Maeda4, Reiner Rincón-Rosales1, Federico Antonio Gutiérrez-Miceli1, Víctor Manuel Ruíz-Valdiviezo1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2729-2743, 2025, DOI:10.32604/phyton.2025.068176 - 30 September 2025

    Abstract Species of the genus Paenibacillus, especially those from extreme environments that have been reported, are known for producing bioactive compounds with agricultural and biotechnological applications. In this study, we investigated the genomic and biochemical potential of Paenibacillus sp. VCA1 strain isolated from a thermophilic environment. Taxonomic identification was performed using whole genome similarity analysis, TETRA four-nucleotide frequency of occurrence analysis, ANI average nucleotide identity analysis, and gene distance analysis using digital DNA-DNA hybridization (dDDH). Functional analysis of the strain VCA1 was performed by detecting genes, enzymes, and genome subsystems involved in biocontrol and plant growth promotion,… More >

  • Open Access

    ARTICLE

    Acalypha gaumeri: Antifungal Activity of Three Populations under Edaphic and Seasonal Variations and Ex-Situ Propagation

    Arely A. Vargas-Díaz1, Daisy Pérez-Brito2, Beatriz Hernández-Carlos3, Jairo Cristóbal-Alejo4,*, Silvia Andrade-Canto2, Marcela Gamboa-Angulo2,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2839-2853, 2025, DOI:10.32604/phyton.2025.066682 - 30 September 2025

    Abstract In the search for new alternatives to control tropical fungal pathogens, the ethanol extracts (EEs) from Acalypha gaumeri (Euphorbiaceae) roots showed antifungal properties against several tropical fungal phytopathogens. A. gaumeri is classified as endemic to the Yucatan Peninsula, Mexico, an area with distinct rainy, drought and northern seasons. The present study evaluated the antifungal activity of three wild populations of A. gaumeri collected quarterly in different seasons during one year against Alternaria chrysanthemi, Colletotrichum gloeosporioides, and Pseudocercospora fijiensis and explored their ex-situ propagation. The highest activity was shown by the EE from the Tinum wild population during the rainy season against A. chrysanthemi,… More >

  • Open Access

    ARTICLE

    Leveraging the WFD2020 Dataset for Multi-Class Detection of Wheat Fungal Diseases with YOLOv8 and Faster R-CNN

    Shivani Sood1, Harjeet Singh2,*, Surbhi Bhatia Khan3,4,5,*, Ahlam Almusharraf6

    CMC-Computers, Materials & Continua, Vol.84, No.2, pp. 2751-2787, 2025, DOI:10.32604/cmc.2025.060185 - 03 July 2025

    Abstract Wheat fungal infections pose a danger to the grain quality and crop productivity. Thus, prompt and precise diagnosis is essential for efficient crop management. This study used the WFD2020 image dataset, which is available to everyone, to look into how deep learning models could be used to find powdery mildew, leaf rust, and yellow rust, which are three common fungal diseases in Punjab, India. We changed a few hyperparameters to test TensorFlow-based models, such as SSD and Faster R-CNN with ResNet50, ResNet101, and ResNet152 as backbones. Faster R-CNN with ResNet50 achieved a mean average precision More >

  • Open Access

    ARTICLE

    Aphicidal and Antimicrobial Activities of Salvia rosmarinus Essential Oil and Its Major Compound, 1,8-Cineole

    Ghizlane Houzi1, Aimad Allali2,3,*, Amine Elbouzidi4,*, Mohamed Taibi4, Mohamed Chebaibi3,5, Ben Khada Zineb6, Ramzi A. Mothana7, Mohammed F. Hawwal7, Rachid Flouchi3,8, Abdeslam Asehraou9, Amal Lahkimi2, Soad Khal-Layoun1

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1239-1251, 2025, DOI:10.32604/phyton.2025.063021 - 30 April 2025

    Abstract This work uses GC-MS to analyze the bioactive compounds of Salvia rosmarinus essential oils (SREO) and evaluates their antibacterial, antifungal, and insecticidal effects, as well as the major component, 1,8-cineole. Chemical analysis identified 16 compounds accounting for 99.19% of the oil’s total content, with 1,8-cineole (33.17%), camphor (16.53%), α-pinene (14.46%), and camphene (8.14%) as the major constituents. Antimicrobial activities were assessed against pathogenic strains using minimal inhibit concentration (MIC) and minimum bactericidal concentration (MBC) assays. SREO exhibited a minimum MIC of 0.128% against P. aeruginosa, while 1,8-cineole showed a minimum MIC of 2.06% against the same strain,… More >

  • Open Access

    ARTICLE

    Exploring the Potential of Locally Sourced Fungal Chitosan for Paper Mechanical Property Enhancement

    Ulla Milbreta1,2, Laura Andze1, Juris Zoldners1, Ilze Irbe1, Marite Skute1, Inese Filipova1,*

    Journal of Renewable Materials, Vol.13, No.3, pp. 583-597, 2025, DOI:10.32604/jrm.2024.057663 - 20 March 2025

    Abstract This study investigated the potential of locally sourced mushrooms as a sustainable alternative to marine-derived chitosan in papermaking. Chitosan was extracted from four local (Boletus edulis, Suillus luteus, Leccinum aurantiacum, Suillus variegatus), one commercially available (Agaricus bisporus) and one laboratory-grown (Phanerochaete chrysosporium) fungal species. Paper handsheets were prepared using either 100% regenerated paper or a 50/50 blend of regenerated paper and hemp fibres. 2.5% chitosan (based on dry mass) was incorporated into the paper mass, using chitosan sourced from B. edulis, A. bisporus, P. chrysosporium, and crustacean chitosan. Fungal chitosan sources were selected based on multiple factors. B. edulis exhibited the highest chitosan yield… More >

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