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  • Open Access

    ARTICLE

    Taguchi Design of the Experimental approach to Increase the Biomass and Cell Wall Chitosan Contents of Zygomycetous Dimorphic Fungus Benjaminiella Poitrasii

    S.R. MANE1,2, E. K. PATHAN3, G. PATIL4, S. G. TUPE5, V. GHORMADE4, B. P. CHAUDHARI1,2, M.V. DESHPANDE5,*

    Journal of Polymer Materials, Vol.38, No.3-4, pp. 219-230, 2021, DOI:10.32381/JPM.2021.38.3-4.4

    Abstract A dimorphic fungus Benjaminiella poitrasii contains high chitin/chitosan (35% of the cell wall) in the mycelial (M) form than its yeast (Y) form (20% of the cell wall). However, the relative proportion of chitosan is more in yeast form cells (chitosan: chitin ratio, 6:1) than mycelial cells (chitosan: chitin ratio, 3:1). Using the Taguchi design of experimental (DOE) approach, interactions among eight different parameters showed that carbon source (starch, 10 g/L), incubation time (48 h), inoculum (M and Y mixed 10%), yeast extract (6 g/L) and peptone (10 g/L), were optimum for maximum biomass production. Under these conditions, the chitosan… More >

  • Open Access

    ARTICLE

    Evaluation of the Antibacterial and Antifungal Capacity of Nanoemulsions Loaded with Synthetic Chalcone Derivatives Di-Benzyl Cinnamaldehyde and Benzyl 4-Aminochalcone

    Flavia Oliveira Monteiro da Silva Abreu1,2,*, Taysse Holanda1, Joice Farias do Nascimento1, Henety Nascimento Pinheiro1, Rachel Menezes Castelo1, Hélcio Silva dos Santos3, Thais Benincá4, Patrícia da Silva Malheiros4, Júlio César Sousa Prado5, Raquel de Oliveira Fontenelle5, Maria Madalena de Camargo Forte2

    Journal of Renewable Materials, Vol.12, No.2, pp. 285-304, 2024, DOI:10.32604/jrm.2023.043919

    Abstract With the increase in antimicrobial resistance, it has become necessary to explore alternative approaches for combating and preventing diseases. DB-cinnamaldehyde (CNM) and Benzyl4-amino (B4AM) are bioactive compounds derived from chalcones but with restricted solubility in aqueous media. Nanoemulsions can enhance the solubility of compounds and can be a promising alternative in the development of novel antimicrobials, with reduced side effects and prolonged release. The objective of this study was to evaluate the stability of oil-in-water nanoemulsions loaded with two distinct types of chalcones at two different dosages, to propose a stable formulation with antimicrobial properties. Results showed that nanoemulsions presented… More > Graphic Abstract

    Evaluation of the Antibacterial and Antifungal Capacity of Nanoemulsions Loaded with Synthetic Chalcone Derivatives Di-Benzyl Cinnamaldehyde and Benzyl 4-Aminochalcone

  • Open Access

    ARTICLE

    In Situ Generation of Copper Nanoparticles in Heat-Treated Copper-Containing Masson’s Pine as a Preservative Process for Sawn Timber

    Minting Lai, Guijun Xie*, Wanju Li, Lamei Li, Yongjian Cao

    Journal of Renewable Materials, Vol.11, No.6, pp. 2665-2678, 2023, DOI:10.32604/jrm.2023.027441

    Abstract Heat-treated wood has good dimensional stability, durability, and color, but its susceptibility to fungal growth affects its commercial value. In this study, lumber harvested from mature Masson’s pine (Pinus massoniana Lamb.) was vacuum impregnated with a basic copper salt solution (copper hydroxide, diethanolamine, and polyethylene glycol 200) prior to heat-treatment at 220°C for 3 h. Antifungal properties, surface chemistry, crystal structure and sugar contents were tested, compared with heat treatment alone. The results showed that the samples treated by heating without copper salt treatment showed poor suppression of fungal growth, the copper-impregnated heat-treated wood suppressed (100%) the growth of Botryodiplodia theobromaeMore >

  • Open Access

    ARTICLE

    Development of Molecular Marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

    Maria Babar1, Siddra Ijaz1,*, Imran Ul Haq2, Muhammad Sarwar Khan1

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1289-1300, 2023, DOI:10.32604/phyton.2023.026469

    Abstract We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of MB-CLsRG gene encoding protein… More >

  • Open Access

    ARTICLE

    Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

    Henry López-López1, Nancy Ruiz-Lau2, Rocío Meza-Gordillo1, Víctor Manuel Ruiz-Valdiviezo1, Joseph Galthier Robledo-Luchetti1, Carlos Alberto Lecona-Guzmán1, Juan José Villalobos-Maldonado1, Luc Dendooven3, Joaquín Adolfo Montes-Molina1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1235-1255, 2023, DOI:10.32604/phyton.2023.025716

    Abstract The objective of this work was to evaluate the effect of Beauveria bassiana (Bb 1205) on controlling Fusarium oxysporum f. sp. lycopersici (Fol 17108) in tomato plants in greenhouse conditions. Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymes β-1,3-glucanases and chitinases. Inoculation of Bb 1205 occurred at a concentration of 1 × 108 conidia·mL−1, which was administered onto the leaves, directly into the soil and via injection. Infection with Fol 17108 occurred with 1 × 106 spores·mL−1, which were added directly to the soil. Spectrophotometry was used for… More >

  • Open Access

    ARTICLE

    In Vitro Anti-Bacterial and Anti-Fungal Activities of Extracts from Different Parts of 7 Zingiberaceae Plants

    Lixian Wu1,#, Yongquan Li1,#, Qiaoguang Li2, Jingxuan Li1, Qinghua Lao1, Yucheng Zhong1, Hui Zhang1,*, Xiu Hu1,*

    Journal of Renewable Materials, Vol.11, No.2, pp. 975-989, 2023, DOI:10.32604/jrm.2022.023547

    Abstract This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family. The inhibitory rate, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of leaf and stem, and root and rhizome extracts from Alpinia katsumadai Hayata, Alpinia oxyphylla Miq × Alpinia henryi K. Schumann, Alpinia oblongifolia Hayata, Alpinia nigra (Gaertn.) Burtt, Amomum villosum Lour, Alpinia zerumbet (Pers.) Burtt. et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method. The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi. At a… More >

  • Open Access

    ARTICLE

    Arbuscular Mycorrhizal Fungal Colonization at Different Succession Stages in Songnen Saline-Alkali Grassland

    Yajie Liu, Yunhui Zhou, Linlin Fang, Chunxue Yang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 297-310, 2023, DOI:10.32604/phyton.2022.023152

    Abstract

    Arbuscular mycorrhizal (AM) fungi can form symbiosis with 90% of the vascular plants and play important roles in ecosystem. To realize the AM fungal colonization at different succession stages in saline-alkali land and screen AM fungi species with great functions, roots and soil samples were collected from the three succession stages of Songnen saline-alkali grassland. The soil properties and AM fungal colonization were measured, and the fungus distributed extensively in three stages was annotated by sequencing for AML1/AML2 target, subsequently, maize was selected as the host to verify its colonization. The results showed that the soil properties improved with the… More >

  • Open Access

    ARTICLE

    Arbuscular Mycorrhizal Fungal Diversity Associated with Olea europaea L. Growing in Yunnan Province, Southwestern China

    Yuebo Jing*, Jihua Mao, Rongbo Li

    Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2719-2732, 2022, DOI:10.32604/phyton.2022.021989

    Abstract Olive (Olea europaea L.) is one of the most important and widely cultivated fruit trees, with high economic, ecological, cultural and scientific value. China began introducing and cultivating olive in the 1960s, and Yunnan Province is one of the main growing areas. Improving the cultivation and productivity of this tree crop species is an important challenge. Olive is a typical mycotrophic species and the potential of arbuscular mycorrhizal fungi (AMF) for this plant is well recognized; nevertheless, studies of olive AMF in China are still very limited. Roots and rhizosphere soils of olive were sampled from five representative growing sites… More >

  • Open Access

    ARTICLE

    Innovative Fungal Disease Diagnosis System Using Convolutional Neural Network

    Tahir Alyas1,*, Khalid Alissa2, Abdul Salam Mohammad3, Shazia Asif4, Tauqeer Faiz5, Gulzar Ahmed6

    CMC-Computers, Materials & Continua, Vol.73, No.3, pp. 4869-4883, 2022, DOI:10.32604/cmc.2022.031376

    Abstract Fungal disease affects more than a billion people worldwide, resulting in different types of fungus diseases facing life-threatening infections. The outer layer of your body is called the integumentary system. Your skin, hair, nails, and glands are all part of it. These organs and tissues serve as your first line of defence against bacteria while protecting you from harm and the sun. The It serves as a barrier between the outside world and the regulated environment inside our bodies and a regulating effect. Heat, light, damage, and illness are all protected by it. Fungi-caused infections are found in almost every… More >

  • Open Access

    ARTICLE

    Significant changes in arbuscular mycorrhizal community and soil physicochemical properties during the saline-alkali grassland vegetation succession

    YAJIE LIU, LINLIN FANG, CHUNXUE YANG*

    BIOCELL, Vol.46, No.11, pp. 2475-2488, 2022, DOI:10.32604/biocell.2022.021477

    Abstract Arbuscular mycorrhizal (AM) fungi are widely distributed in various habitats, and the community composition varies in response to the changing environmental conditions. To explore the response of community composition to the succession of saline-alkali land, soil samples were collected from three succession stages of Songnen saline-alkali grassland. Subsequently, the soil characteristics were determined and the AM fungi in soil samples were analyzed by high-throughput sequencing. Then, the response relationship between community composition and soil characteristics was studied by Canonical correlation and Pearson analyses. The soil properties improved with the succession of saline-alkali grassland. There was no significant difference in alpha… More >

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