Abdel Nasser H. Zaied¹, Ehab Rushdy², Mona Gamal^3,*

Computer Systems Science and Engineering, Vol.47, No.2, pp. 1349-1364, 2023, DOI:10.32604/csse.2023.033596

Abstract Acute leukemia is an aggressive disease that has high mortality rates worldwide. The error rate can be as high as 40% when classifying acute leukemia into its subtypes. So, there is an urgent need to support hematologists during the classification process. More than two decades ago, researchers used microarray gene expression data to classify cancer and adopted acute leukemia as a test case. The high classification accuracy they achieved confirmed that it is possible to classify cancer subtypes using microarray gene expression data. Ensemble machine learning is an effective method that combines individual classifiers to classify new samples. Ensemble classifiers… More >

Xiaolan Guo^1,2, Jinbin Hu³, Shimei Yang⁴, Delu Wang^2,*, Jianbing Wang¹

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2683-2697, 2023, DOI:10.32604/phyton.2023.029820

Abstract The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family… More >

Tongman Zhao^1,2,#, Yujie Fan^1,2,#, Yabing Cao^1,2, Xiaogai Zhao^1,2, Guoqiang Fan^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2473-2488, 2023, DOI:10.32604/phyton.2023.029469

Abstract Somatic embryogenesis receptor-like kinases (SERKs) are receptor-like proteins that contain leucine-rich repeats and are involved in various signaling pathways. This study identified SERK family members in the Paulownia fortunei genome and analyzed their characteristics and expression profiles using bioinformatics methods. We identified 12 SERK genes with relatively conserved gene structures and motifs that were distributed unevenly on eight Paulownia chromosomes. The gene promoters contained various cis-acting elements that regulated the expression of the PfSERK genes in response to hormones and abiotic stresses. Synteny analysis indicated that 10 segmental duplication events had occurred during evolution of the PfSERK family. The expression… More >

Chongpei Zheng¹, Liangliang Li², Zhencheng Han², Weijie Li^2,*, Xiaopeng Wen^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2399-2412, 2023, DOI:10.32604/phyton.2023.029891

Abstract

To explore how manganese affects the antioxidant system and the expression levels of related genes of “Hong yang” seedlings, the leaves of its tissue cultured seedlings were taken as test materials, and single factor treatment was performed by changing the manganese chloride (MnCl₂^.4H₂O) solution concentration when spraying the leaves. The expression levels of Mn-SOD, POD64 and POD27 genes in leaves were quantitatively analyzed by real-time quantitative PCR (qRT-PCR) at different determination times. Meanwhile, the contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), the activities of antioxidant enzymes, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The results showed… More >

Ali Bandehagh^1,*, Zahra Dehghanian², Vahideh Gougerdchi¹, Mohammad Anwar Hossain^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2301-2324, 2023, DOI:10.32604/phyton.2023.028586

Abstract The presence of selenium (Se) is not widely established as crucial for crops, although it is commonly recognized as an important nutrient for animals as well as humans. Even so, it is inevitably accepted that Se usually contributes positively to the life cycle of plants. Previous findings suggested that small amounts of Se seem to have a productive role in growth and production. As a result, Se is assumed to function in multiple ways, primarily by influencing a variety of biochemical and physiological functions. Also, Se also acts as a plant antioxidant and pro-oxidant and confers tolerance against different abiotic… More >

Cong Hou¹, Kang Ning¹, Xiuye Wei¹, Yufei Cheng¹, Huatao Yu¹, Haibin Yu², Xia Liu^1,*, Linlin Dong^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2087-2103, 2023, DOI:10.32604/phyton.2023.029041

Abstract Abscisic acid (ABA) is involved in regulating diverse biological processes, but its signal transduction genes and roles in hemp seed germination are not well known. Here, the ABA signaling pathway members, PYL, PP2C and SnRK2 gene families, were identified from the hemp reference genome, including 7 CsPYL (pyrab-actin resistance1-like, ABA receptor), 8 CsPP2CA (group A protein phosphatase 2c), and 7 CsSnRK2 (sucrose nonfermenting1-related protein kinase 2). The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage. Exogenous ABA (1 or 10 μM) treatment had a significant regulatory effect on the selected PYL, PP2C,… More >

Talha Ali Chohan^1,*, Tahir Ali Chohan^2,*, Muhammad Zahid Mumtaz¹, Muhammad Waqar Alam³, Salah ud Din⁴, Iqra Naseer⁵, Ayesha Riaz¹, Tayyeba Naseem¹, Areeba Iftikhar⁶, Dur E. Najaf Ali¹, Mubashir Hassan⁷, Hayssam M. Ali⁸

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 1943-1954, 2023, DOI:10.32604/phyton.2023.026945

Abstract Myzus persicae (M. persicae) is now considered a threat to agricultural crops due to economic losses. Numerous synthetic insecticides applied every year against M. persicae, are reported to be unsafe for environment, humans, and beneficial insects. Furthermore, several species of Myzus have been found to develop resistance due to over application of these insecticides. Therefore, it is required to find some novel insecticide that would be safe for the environment as well as for humans. In the current study, two major pure constituents α-pinene and β-caryophyllene were evaluated for their insecticidal potential against M. persicae using a fumigant toxicity assay.… More >

Peiling Wang^1,#, Xiaohong Xu^2,#, Yong Li², Hecui Zhang¹, Xuejie Zhang³, Siru Zhou¹, Yimei Liu¹, Yunyan Feng¹, Tonghong Zuo¹, Liquan Zhu^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1469-1492, 2023, DOI:10.32604/phyton.2023.027133

Abstract Phytocyanin (PC) is a class of plant-specific blue copper proteins involved in electron transport, plant growth, development, and stress resistance. However, PC proteins have not been systematically evaluated in tobacco plants. We determined the whole-genome sequences of the PC family in the tobacco cultivar ‘K326.’ The transcriptome data were used to analyze the expression of the NtPC family at different development stages and tissue-specific genes. Real-time fluorescence quantitative analysis was used to analyze the expression of the NtPC gene family under low temperature and methyl jasmonate stress. The tobacco NtPC family contained 110 members and was divided into four subfamilies:… More >

Min Hu^1,2,3, Zhimin Chen⁴, Yuan Xia⁴, Liping Zhang^1,2,3,*, Qiuhua Tang^1,2,3

CMES-Computer Modeling in Engineering & Sciences, Vol.136, No.3, pp. 2815-2840, 2023, DOI:10.32604/cmes.2023.027146

Abstract The multi-skill resource-constrained project scheduling problem (MS-RCPSP) is a significant management science problem that extends from the resource-constrained project scheduling problem (RCPSP) and is integrated with a real project and production environment. To solve MS-RCPSP, it is an efficient method to use dispatching rules combined with a parallel scheduling mechanism to generate a scheduling scheme. This paper proposes an improved gene expression programming (IGEP) approach to explore newly dispatching rules that can broadly solve MS-RCPSP. A new backward traversal decoding mechanism, and several neighborhood operators are applied in IGEP. The backward traversal decoding mechanism dramatically reduces the space complexity in… More > Graphic Abstract

Shoukai Lin^1,2,#, Shichang Xu^1,#, Liyan Huang¹, Fuxiang Qiu¹, Yihong Zheng¹, Qionghao Liu¹, Shiwei Ma^1,2, Bisha Wu^1,2, Jincheng Wu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1185-1201, 2023, DOI:10.32604/phyton.2023.026752

Abstract Loquat (Eriobotrya japonica Lindl.) is a subtropical evergreen fruit tree that produces fruits with abundant nutrients and medicinal components. Confirming suitable reference genes for a set of loquat samples before qRT-PCR experiments is essential for the accurate quantification of gene expression. In this study, eight candidate reference genes were selected from our previously published RNA-seq data, and primers for each candidate reference gene were designed and evaluated. The Cq values of the candidate reference genes were calculated by RT-qPCR in 31 different loquat samples, including 12 subgroups of developing or abiotic-stressed tissues. Different combinations of stable reference genes were screened… More >