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  • Open Access

    ARTICLE

    Comparative Study of Genetic Structure and Genetic Diversity between Wild and Cultivated Populations of Taxus cuspidata, Northeast China

    Dandan Wang, Xiaohong Li, Yanwen Zhang*

    Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 355-369, 2024, DOI:10.32604/phyton.2024.047183

    Abstract Taxus cuspidata is a rare plant with important medicinal and ornamental value. Aiming at the obvious differences between wild and cultivated populations of T. cuspidata from Northeast China, a total of 61 samples, that is, 33 wild yews and 28 cultivated yews were used to analyze the differences and correlations of the kinship, genetic diversity, and genetic structure between them by specific length amplified fragment sequencing (SLAF-seq). Finally, 470725 polymorphic SLAF tags and 58622 valid SNP markers were obtained. Phylogenetic analysis showed that 61 samples were classified into 2 clusters: wild populations and cultivated populations, and some wild yews were… More >

  • Open Access

    ARTICLE

    Population Structure Analysis and Genome-Wide Association Study of Tea (Camellia sinensis (L.) K untze) Germplasm in Qiannan, China, Based on SLAFSeq Technology

    Fen Zhang1, Weili Tian1, Lu Cen1, Litang Lv2, Xiaofang Zeng1, Yulu Chen1, Yichen Zhao2,*, Yan Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 791-809, 2022, DOI:10.32604/phyton.2022.018104

    Abstract Duyun Maojian tea is a famous tea in China. In this study, the specific-locus amplified fragment (SLAF) sequencing method was used to analyze the population structure and conduct a genome-wide association study (GWAS) of 2 leaf traits of 123 tea plants in Qiannan, China. A total of 462,019 SLAF tags and 11,362,041 single-nucleotide polymorphism (SNP) loci were obtained. The results of phylogenetic tree analysis, cluster analysis, and principal component analysis showed that 123 tea germplasms were clustered into three groups, and the heterozygosity rates for Groups I, II, and II were 0.206, 0.224, and 0.34, respectively. Generally, tea germplasms in… More >

  • Open Access

    ARTICLE

    Incidence, genomic diversity, and evolution of strawberry mottle virus in China

    LINGJIAO FAN, CHENGYONG HE, MENGMENG WU, DEHANG GAO, ZHENFEI DONG, SHENGFAN HOU, ZEKUN FENG, HONGQING WANG*

    BIOCELL, Vol.45, No.4, pp. 1137-1151, 2021, DOI:10.32604/biocell.2021.015396

    Abstract Strawberry mottle virus (SMoV) is one of the most common viruses infecting strawberries, causing losses to fruit yield and quality. In this study, 165 strawberry leaf samples were collected from six provinces of China, 46 of which tested positive for SMoV. The complete genome sequences of 11 SMoV isolates were obtained from Liaoning (DGHY3, DGHY16-2, DGHY17, DGHY20-2, DGHY21, DGHY26-2), Shandong (SDHY1, SDHY5, SDHY31-2, SDHY33-2), and Beijing (BJMX7). The RNA1 and RNA2 nucleotide identities between the 11 Chinese isolates were 95.4–99.3% and 96.3–99.6%, respectively, and they shared 78.4–96.6% and 84.8–93.5% identities with the available SMoV isolates in GenBank. Recombination analysis revealed… More >

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