Ling Li^1,*, Xuyu Yan¹, Lei Mei², Shuijin Zhu²

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 315-327, 2020, DOI:10.32604/phyton.2020.09108 - 22 April 2020

Abstract The risk of cotton production on arable land contaminated with heavy metals has increased in recent years. Cotton shows stronger and more extensive resistance to heavy metals, such as cadmium (Cd) than that of other major crops. Here, a potted plant experiment was performed to study Cd-induced alterations in the cottonseed kernel gossypol content and pigment gland structure at maturity in two transgenic cotton cultivars (ZD-90 and SGK3) and an upland cotton standard genotype (TM-1). The results showed that Cd accumulation in cottonseed kernels increased with increasing Cd levels in the soil. The seed kernel… More >

Harika Atmaca¹, Gurbuz Gorumlu², Burcak Karaca², Mustafa Degirmenci², Didem Tunali², Yalcin Cirak², Duygu Unuvar Purcu¹, Selim Uzunoglu¹, Bulent Karabulut², Ulus A. Sanli², Ruchan Uslu²

European Cytokine Network, Vol.20, No.3, pp. 121-130, 2009, DOI:10.1684/ecn.2009.0159

Abstract In the present study, we aimed to evaluate the possible synergistic, cytotoxic effects of combination treatment of gossypol and zoledronic acid, in human ovarian cancer cell lines, OVCAR-3 and MDAH-2774, and to elucidate the role of this novel combination treatment on angiogenesis-related molecules in ovarian cancer. The XTT cell viability assay was used for showing cytotoxicity. Both DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used for demonstrating apoptosis. To elucidate the angiogenic molecules affected by combination treatment, mRNA levels of angiogenic molecules were measured using the Human Angiogenesis RT² Profiler^TM PCR Array… More >

Burcak Karaca¹, Yuksel Kucukzeybek¹, Gurbuz Gorumlu¹, Cigdem Erten¹, Mustafa K. Gul¹, Ercument Cengiz¹, Harika Atmaca², Selim Uzunoglu², Ulus A. Sanli¹, Bulent Karabulut¹, Ruchan Uslu¹

European Cytokine Network, Vol.19, No.4, pp. 176-184, 2008, DOI:10.1684/ecn.2008.0139

Abstract In this study, we aimed to investigate the angiogenic cytokine profiles of hormone- and drug-refractory prostate carcinoma cell lines, PC-3 and DU-145. We also studied the effect of gossypol, a natural polyphenolic cotton-seed extract, on the angiogenic cytokine profile of these cell lines. XTT cell proliferation assay was used for the assessment of cytotoxicity. For apoptosis, both histone-DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used. Angiogenic cytokine profiles of supernatants from both cell lines, before and after treatment with gossypol, were investigated using the human angiogenesis antibody array I^®. It was shown… More >