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    ARTICLE

    Improved Culture Media for Embryogenic Callus Generation in Sorghum [Sorghum bicolor (L.) Moench]

    Paul Chege1,2, Andrea Palágyi2, Csaba Lantos2, Erzsébet Kiss1, János Pauk2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 111-119, 2020, DOI:10.32604/phyton.2020.07554

    Abstract Many attempts on optimization of sorghum [Sorghum bicolor (L.) Moench] tissue culture induction media have been made, but the culture system remains with some bottlenecks compared to that of other crops. This study aimed at assessing the suitability of various induction media to produce embryogenic callus (yellow and friable) with high induction rates and reduced phenolic exudation. The six culture medium modifications: 3 based on Murashige and Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes. Although there was a genotype… More >

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