ANA ROCÍO ROLDÁN PALOMO1, PEDRO MARTÍN1, ALEJANDRO REBOLLEDO1*, NICOLÁS ENRIQUE1, LUIS E. FLORES2, VERÓNICA MILESI1
BIOCELL, Vol.36, No.2, pp. 73-81, 2012, DOI:10.32604/biocell.2012.36.073
Abstract After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine… More >
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