Laura Isabel Arias-Rodríguez¹, Martha Alicia Rodríguez-Mendiola^2,*, Carlos Arias-Castro^2,*, Federico Antonio Gutiérrez Miceli¹, Diana Reséndez Pérez³, María Celina Luján Hidalgo¹, Juan José Villalobos Maldonado¹, Norma Alejandra Mancilla Margalli²

Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2807-2823, 2023, DOI:10.32604/phyton.2023.030396 - 15 September 2023

Abstract In recent years, there has been a growing interest in exploring alternative treatments for bone defects. Bone tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone regeneration. In our study, we specifically investigated the extract of Petroselinum crispum, a plant known for its abundance of osteogenic flavonoids such as apigenin, quercetin, and kaempferol. Our objective was to compare the total flavonoid content (TFC) and their accumulation in different sources. We obtained hydrolyzed aqueous extracts from the leaves of parsley plants (grown for 12 weeks in the field), weekly… More >

JINFANG HU^1,#, JIANGEN AO^2,#, LONGSHENG FU^1,#, YAOQI WU¹, FENG SHAO³, TIANTIAN XU¹, MINGJIN JIANG⁴, SHAOFENG XIONG¹, YANNI LV^1,*

BIOCELL, Vol.47, No.7, pp. 1611-1622, 2023, DOI:10.32604/biocell.2023.028684 - 21 June 2023

Abstract Background: The attenuation function of Dalbergia odorifera leaves on cerebral ischemia-reperfusion (I/R) is little known. The candidate targets for the Chinese herb were extracted from brain tissues through the high-affinity chromatography. The molecular mechanism of D. odorifera leaves on cerebral I/R was investigated. Methods: Serial affinity chromatography based on D. odorifera leaves extract (DLE) affinity matrices were applied to find specific binding proteins in the brain tissues implemented on C57BL/6 mice by intraluminal middle cerebral artery occlusion for 1 h and reperfusion for 24 h. Specific binding proteins were subjected to mass-spectrometry to search for the differentially expressed… More >

Iva Šutevski^1,#, Klara Krmpotić^1,#, Sandra Vitko¹, Nataša Bauer¹, Eva Fancev², Mario Cifrek², Željka Vidaković-Cifrek^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 901-920, 2023, DOI:10.32604/phyton.2023.025165 - 29 November 2022

Abstract Mechanical stimulation of plants can be caused by various abiotic and biotic environmental factors. Apart from the negative consequences, it can also cause positive changes, such as acclimatization of plants to stress conditions. Therefore, it is necessary to study the physiological and biochemical mechanisms underlying the response of plants to mechanical stimulation. Our aim was to evaluate the response of model plant Arabidopsis thaliana to a moderate force of 5 N (newton) for 20 s, which could be compared with the pressure caused by animal movement and weather conditions such as heavy rain. Mechanically stimulated leaves… More >

Xiaoming Tian^*, Guangfeng Xiang, Hao Lv, Jing Peng, Lu Zhu

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 837-850, 2023, DOI:10.32604/phyton.2023.024408 - 29 November 2022

Abstract In the present study, the variation patterns of leaf shape in different populations of individual Semiliquidambar cathayensis plants were analyzed to investigate the relationship among leaf shape variation, photosynthetic properties, and active compounds to understand the genetic characteristics of S. cathayensis and screen elite germplasms. The leaf shape of 18 offspring from three natural S. cathayensis populations was analyzed to investigate the level of diversity and variation patterns of leaf shape. Furthermore, photosynthetic pigment content, physiological parameters of photosynthesis, and the active compounds in leaves of different shapes were determined. Statistical analysis showed that the leaf shape variation in  S.… More >

Masayuki Honda¹, Kenichi Tatsumi^2,*, Masaki Nakagawa³

CMES-Computer Modeling in Engineering & Sciences, Vol.133, No.3, pp. 557-577, 2022, DOI:10.32604/cmes.2022.020623 - 03 August 2022

Abstract A model to predict photosynthetic carbon assimilation rate (A) with high accuracy is important for forecasting crop yield and productivity. Long short-term memory (LSTM), a neural network suitable for time-series data, enables prediction with high accuracy but requires mesophyll variables. In addition, for practical use, it is desirable to have a technique that can predict A from easily available information. In this study, we propose a BLSTMaugmented LSTM (BALSTM) model, which utilizes bi-directional LSTM (BLSTM) to indirectly reproduce the mesophyll variables required for LSTM. The most significant feature of the proposed model is that its hybrid… More >

Zhiguo Lu¹, Yuhong Zheng^1,*, Pengchong Zhang², Boyuan Fan³, Aimin Yu⁴, Li Fu^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2549-2558, 2022, DOI:10.32604/phyton.2022.021288 - 12 July 2022

Abstract In this communication, we used electrochemical sensor for recording the electrochemical profiles of eleven species of Yulania spp. from leaf extract. Two solvents and two buffer conditions were used for electrochemical fingerprints collection. Their electrochemical fingerprints can be converted to different patterns and consequently for species recognition. The results indicate the pattern recognition is much convenient than that of the recognition of species directly using voltammetric signal. The current information in electrochemical fingerprinting represents the type and amount of electrochemically active molecules, which linked to the genetic differences among the plants. Therefore, the electrochemical fingerprints were More >

Kuntal Das¹, Syed Mohammed Basheeruddin Asdaq^2,*, M. Saifulla Khan^1,*, Sravani Singirikonda¹, Abdulhakeem S. Alamri^3,4, Walaa F. Alsanie^3,4, Majid Alhomrani^3,4, Sreeharsha Nagaraja^5,6, Katharigatta N. Venugopala^5,7

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2349-2365, 2022, DOI:10.32604/phyton.2022.022379 - 30 May 2022

Abstract The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence… More >

Nouh Sabri Elmitwally^1,2, Maria Tariq^3,4, Muhammad Adnan Khan^5,*, Munir Ahmad³, Sagheer Abbas³, Fahad Mazaed Alotaibi⁶

Computer Systems Science and Engineering, Vol.42, No.3, pp. 1001-1014, 2022, DOI:10.32604/csse.2022.022017 - 08 February 2022

Abstract In the agricultural industry, rice infections have resulted in significant productivity and economic losses. The infections must be recognized early on to regulate and mitigate the effects of the attacks. Early diagnosis of disease severity effects or incidence can preserve production from quantitative and qualitative losses, reduce pesticide use, and boost ta country’s economy. Assessing the health of a rice plant through its leaves is usually done as a manual ocular exercise. In this manuscript, three rice plant diseases: Bacterial leaf blight, Brown spot, and Leaf smut, were identified using the Alexnet Model. Our research More >

Yuting Luan¹, Cong Xu¹, Xiaoxiao Wang¹, Daqiu Zhao¹, Jun Tao^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1045-1059, 2022, DOI:10.32604/phyton.2022.018767 - 24 January 2022

Abstract The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes, ACT1, ACT2, GAPDH, eIF1, eIF2, α-TUB, β-TUB, TBP, RNA Pol II and RP II, were calculated for a suitable reference gene selection in Paeonia ostii T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed More >

IVY LEUNG¹, MARIA-LUISA VEISAGA², MARGARITA ESPINAL¹, WEI ZHANG¹, ROBERT BARNUM¹, MANUEL ALEJANDRO BARBIERI^1,2,3,4,*

BIOCELL, Vol.46, No.3, pp. 567-578, 2022, DOI:10.32604/biocell.2022.014009 - 18 November 2021

Abstract The Annona genus is a member of Annonaceae, one of the largest families of plants across tropical and sub-tropical regions. This family has been used in several ethnomedicinal practices to treat a multitude of human diseases. However, the molecular mechanism underlying its effect on the lipid droplet formation and on the expression of adipogenic markers of this plant remain to be investigated. In this study, we examined whether the extracts from the aerial part of Annona montana affect in vitro differentiation of preadipocytes. For our investigations, both mouse embryo fibroblast 3T3-L1 and normal human primary subcutaneous preadipocytes were… More >