ALI M.SHARIFI1,2*, RADBOD DARABI2, AND KHOSROW JADIDI3
BIOCELL, Vol.34, No.1, pp. 53-55, 2010, DOI:10.32604/biocell.2010.34.053
Abstract The effects of human versus mouse EGF on cell growth and culture duration were studied to optimize a human limbal stem cells culture method for therapeutical autologous transplantation. Limbal cells were obtained by trypsin digestion and transferred to a culture medium. The time needed to reach full confluence in culture was determined. Specific antibodies to corneal stem cell marker (P63) versus corneal epithelial differentiation marker (K3) were used for histochemical determinations. A high proportion of P63 positive cells (85± 4.6%), and a correspondingly low proportion K3 positive cells (15 ± 3.8%) indicated that most cultured cells remained undifferentiated and were… More >
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