DONGMEI GAO^1,#, JIAOE CHEN^2,#, HONGZHANG LI^2,*, JUN ZHAO^3,*

BIOCELL, Vol.46, No.10, pp. 2275-2280, 2022, DOI:10.32604/biocell.2022.021008 - 13 June 2022

Abstract Introduction: Human cytomegalovirus (HCMV) is reported to be involved in the occurrence of many human diseases. To further investigate the biological changes of HCMV, we analyzed the relevant factors that affect the autophagy caused by HCMV infection. Methods: Firstly, we cultured human embryonic lung fibroblasts (HELF) cells with HCMV infection, and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay (ELISA), Real-time quantitative Polymerase Chain Reaction (RT-qPCR), Acridine orange (AO) staining and Western blotting (WB) experiments. Results: Through the above experiments, we found that the combined treatment of HCMV infection and carbamazepine, More >

YAPING WANG^1,2,#,*; XIAOHONG XUE^2,#; ZHEN ZHAO^1,#; XIAOLIN LI²; ZHIYONG ZHU¹

BIOCELL, Vol.46, No.7, pp. 1651-1659, 2022, DOI:10.32604/biocell.2022.018559 - 17 March 2022

Abstract The pathogenesis of high altitude-related gastric mucosal injury remains poorly understood, this study aimed to investigate the role of autophagy in hypoxia-induced apoptosis of rat gastric mucosal cells. Rats were randomized into four groups which were maintained at an altitude of 400 m (P) or received no treatment (H), autophagy inducer rapamycin (H+AI) or autophagy inhibitor 3-MA (H+AB) at an altitude of 4,300 m for 1, 7, 14 and 21 days, respectively, and the morphology, ultrastructure, autophagy, and apoptosis of gastric mucosal tissues were examined. Gastric mucosal epithelial cells CC-R039 were cultured under conditions of… More >

RONGXUE WEI¹, CHUNCHUN HAN^1,*, FENGJIANG YE¹, SHOUHAI WEI², FANG HE¹, HEHE LIU¹, LIANG LI¹, HONGYONG XU¹, SHENQIANG HU¹, XIANYIN ZENG²

BIOCELL, Vol.46, No.1, pp. 171-183, 2022, DOI:10.32604/biocell.2022.015409 - 28 September 2021

Abstract In order to explore the role of forkhead box protein O1 (FoxO1) in the lipid metabolism and cell proliferation, goose primary hepatocytes were isolated and incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, and then transfected with FoxO1 interference plasmid. The related parameters of lipid metabolism and cell proliferation were measured. The results firstly showed that FoxO1 interference increased the intracellular TG and lipids concentration (P < 0.05); and increased the proliferative index (PI), cell DNA synthesis, protein expression of Cyclin D1 in goose primary hepatocytes (P < 0.05). Secondly, the co-treatment of insulin and… More >

JIAFU FENG^1,2,#,*, BEI XU^1,2,#, CHUNMEI DAI^1,2,#, YAODONG WANG^1,4, GANG XIE^1,5, WENYU YANG^1,2, BIN ZHANG^1,2, XIAOHAN LI⁶, JUN WANG³

Oncology Research, Vol.29, No.5, pp. 331-349, 2021, DOI:10.32604/or.2022.03554 - 10 October 2022

Abstract Due to its difficulty in early diagnosis and lack of sensitivity to chemotherapy and radiotherapy, renal cell carcinoma (RCC) remains to be a frequent cause of cancer-related death. Here, we probed into new targets for its early diagnosis and treatment for RCC. microRNA (miRNA) data of M2-EVs and RCC were searched on the Gene Expression Omnibus database, followed by the prediction of the potential downstream target. Expression of target genes was measured via RT-qPCR and Western blot, respectively. M2 macrophage was obtained via flow cytometry with M2-EVs extracted. The binding ability of miR-342-3p to NEDD4L… More >

Kazuhiro Yamamoto^*, Takeshi Ioroi^*, Kazuaki Shinomiya^†, Ayaka Yoshida^*, Kenichi Harada^‡, Masato Fujisawa^‡, Tomohiro Omura^*, Yasuaki Ikemi^§, Shunsaku Nakagawa^§, Atsushi Yonezawa^§, Osamu Ogawa^¶, Kazuo Matsubara^§, Takuya Iwamoto^#, Kohei Nishikawa^**, Sayaka Hayashi^††, Daichi Tohara^††, Yoji Murakami^‡‡, Takanobu Motoshima^‡‡, Hirofumi Jono^††, Ikuko Yano^*§

Oncology Research, Vol.29, No.1, pp. 11-23, 2021, DOI:10.3727/096504022X16418911579334

Abstract We evaluated the association of signal transducer and activator of transcription 3 (STAT3) polymorphisms with the incidence of mammalian target of rapamycin (mTOR) inhibitor-induced interstitial lung disease (ILD) in patients with renal cell carcinoma (RCC). We also used lung-derived cell lines to investigate the mechanisms of this association. Japanese patients with metastatic RCC who were treated with mTOR inhibitors were genotyped for the STAT3 polymorphism, rs4796793 (−1697C/G). We evaluated the association of the STAT3 genotype with the incidence of ILD and therapeutic outcome. In the 57 patients included in the primary analysis, the ILD rate… More >

LIETING MA¹, MIAOLING LI^2,*, XINGLONG ZHENG³

BIOCELL, Vol.45, No.2, pp. 317-321, 2021, DOI:10.32604/biocell.2020.09313 - 19 February 2021

Abstract Ovarian cancer is a malignant cancer type and affects women’s lives in the world. Circular RNAs (circRNAs) have been involved with the progression of cancers. In our study, we are going to explore the functions of circATF6 in ovarian cancer. The qRT-PCR assay was used to detect expressions of genes. Actinomycin D and RNase R treatment were implemented to verify the circular RNA character of circATF6. Besides, Cell proliferation was assessed by colony formation assay and EdU assay. Silenced circATF6 could reduce the proliferation of ovarian cancer cells. In addition, inhibited circATF6 could promote the More >

PENG WU^#, MANLI GAO^#, JIANJIAN DONG, CHENCHEN XU, BO LI, XUN WANG, YONGZHU HAN, NAN CHENG^*

BIOCELL, Vol.45, No.1, pp. 109-117, 2021, DOI:10.32604/biocell.2021.012048 - 26 January 2021

Abstract Wilson disease (WD), known as hepatolenticular degeneration (HLD), is a treatable autosomal recessive disorder of copper metabolism. Because copper deposits in the liver first, the liver is not only the original defective organ but also the most affected organ. The liver injury is also one of the main causes of death throughout the course of the disease. Therefore, the treatment of liver injury is the main task of WD treatment, which is of great significance to improve the prognosis of patients. Autophagy is a process that promotes cell survival through degradation, recycling, and absorption in… More >

ATTALLA EL-KOTT^1,2, EMAN ELBEALY³, FAHMY ELSAID^1,4, HAITHAM EL-MEKKAWY¹, ABD-EL-KARIM ABD-LATEIF⁵, ABDULALI TAWEEL⁶, HEBA KHALIFA², AHMAD KANDEEL⁵, KAREEM MORSY^1,7, ESSAM IBRAHIM^1,8,9, MASHAEL MOHAMMED BIN-MEFERIJ^10,*

BIOCELL, Vol.45, No.1, pp. 89-101, 2021, DOI:10.32604/biocell.2021.012516 - 26 January 2021

Abstract This study investigates if the anti-tumor effect of Pterostilbene in the SKOV3 ovarian cancer (OC) cell line involves inhibition of cell metabolism and tested in this effect involves modulating AMPK and Akt-induced regulation of mTORC1. Initially, SKOV3 cells were cultured in the humidified conditions in DMEM media for 24 h with or without increasing concentration of Pterostilbene. Then, the cells were incubated with Pterostilbene (IC₅₀ = 50 µM) under similar conditions with or without pre-incubation with Dorsomorphin, an AMPK inhibitor. In a dose-dependent manner, Pterostilbene inhibited SKOV3 cell survival and increased their lysate levels of lactate… More >

Huasong Liu^*1, Jun Zhang^*1, Xiangyu Luo^*, Min Zeng^*, Liqiang Xu^*, Qunxian Zhang^*, Hua Liu^*, Jialong Guo^*, Lanlan Xu^†

Oncology Research, Vol.28, No.1, pp. 65-73, 2020, DOI:10.3727/096504019X15656904013079

Abstract Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) mediate the development of esophageal squamous cell carcinoma (ESCC) via various pathophysiological pathways. This study explored the impact of the lncRNA FOXD2-AS1 on cisplatin resistance in ESCC and its possible mechanisms. Upregulation of FOXD2-AS was detected in patients with ESCC and ESCC cells that are resistant to cisplatin. In an in vitro assay, knockdown of FOXD2-AS1 noticeably inhibited cell invasion and growth, triggered cell death, and repressed the stimulation of the Akt/mTOR axis in cisplatin-resistant ESCC cells (TE-1/DDP). Conversely, the overexpression of FOXD2-AS1 remarkably increased cell More >

Majed Al Fayi^*†, Ahmad Alamri^*, Prasanna Rajagopalan^*†

Oncology Research, Vol.28, No.2, pp. 177-189, 2020, DOI:10.3727/096504019X15746768080428

Abstract Drug discovery research to fight lung cancer is incessantly challenged by drug resistance. In this study, we used drug-resistant lung cancer stem like cells (A549-CS) to compare the efficacy of standard drugs like cisplatin (DDP) and gemcitabine (GEM) with a novel arylidene derivative IOX-101. A549-CS was derived from regular A549 cells by growing in special media. Resistance proteins were detected using Western blotting. Cell proliferations were assessed by MTT assay. Cytokine release was enumerated using enzyme-linked immunosorbent assay. The effect of drugs on apoptosis and cell cycle was studied with flow cytometry protocols. Apoptosis-related proteins,… More >