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  • Open Access

    ARTICLE

    Genetic diversity of water primrose (Ludwigia hyssopifolia) in Thailand based on morphological characters and RAPD analysis

    Tantasawat PA, K Lunwongsa, T Linthaisong, P Wirikitgul, N Campatong, N Talpolkrung, A Tharapreuksapong, O Poolsawat, A Khairum, A Sorntip, C Kativat

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 162-170, 2018, DOI:10.32604/phyton.2018.87.162

    Abstract Genetic diversity and relatedness of 17 water primrose (Ludwigia hyssopifolia) accessions in Thailand were estimated using morphological characters and random amplified polymorphic DNA (RAPD) markers. Eight morphological characters were diverse among the accessions. However, some accessions could not be distinguished from one another based on these morphological characters alone. Unweighted pair-group arithmetic average (UPGMA) analysis of these characters separated these 17 accessions into 2 major clusters. Among the 5 RAPD primers used, a total of 68 fragments (150 to 2000 bp) were amplified, showing a polymorphism percentage of 80%. The polymorphic information content (PIC) among accessions varied from 0.31 to… More >

  • Open Access

    ARTICLE

    Molecular differentiation of Xoconostle (Opuntia spp.) using AFLP molecular markers

    García-Zambrano EA1, MC Ojeda-Zacarías1, JG Gutiérrez-Soto1, SR Sinagawa-García1, A Gutiérrez-Díez1, J Martínez-de la Cerda1, JA Pedroza-Flores1, C Gallegos-Vázquez2, JI García-Zambrano3

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

    Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >

  • Open Access

    ARTICLE

    Kaempferol and docetaxel diminish side population and down-regulate some cancer stem cell markers in breast cancer cell line MCF-7

    SARA SOLTANIAN1, HELIA RIAHIRAD1, ATHAREH PABARJA1, MOHAMMAD REZA KARIMZADEH2, KOLSOUM SAEIDI3,4

    BIOCELL, Vol.41, No.2-3, pp. 33-40, 2017, DOI:10.32604/biocell.2017.41.033

    Abstract Cancer stem cells (CSCs) are a small subpopulation of cancer cells whose resistance to chemotherapy and radiotherapy plays a pivotal role in treatment failure, tumor recurrence and metastasis. Today, the ability of many phytochemicals in targeting of CSCs has been identified. Kaempferol is a plant phytoestrogen that was recognized as a useful agent for targeting cancer cells by apoptosis induction, cell cycle arrest and inhibition of angiogenesis, migration, and invasion of cancer cells. In this study, we compared the effect of docetaxel as a common breast cancer chemotherapy drug and kaempferol on MCF-7 breast CSCs. Results showed that both docetaxel… More >

  • Open Access

    ARTICLE

    Review : Biochemical-molecular markers in unilateral ureteral obstruction

    WALTER MANUCHA

    BIOCELL, Vol.31, No.1, pp. 1-12, 2007, DOI:10.32604/biocell.2007.31.001

    Abstract Congenital obstructive nephropathy is the primary cause of end-stage renal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstructive uropathy effects -decline in the plasmatic renal flow and glomerular filtration rate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosis- are a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-β (TGF-β) and nitric oxide (NO) have been… More >

  • Open Access

    ARTICLE

    An alternating finite difference material point method for numerical simulation of high explosive explosion problems

    X. X. Cui1, X. Zhang1,2, K. Y. Sze3, X. Zhou4

    CMES-Computer Modeling in Engineering & Sciences, Vol.92, No.5, pp. 507-538, 2013, DOI:10.3970/cmes.2013.092.507

    Abstract Based on the material point method (MPM), an alternating finite difference material point (AFDMP) method is proposed for modeling the 3D high explosive (HE) explosion and its interaction with structures nearby. The initiatory detonation and eventual fluid structure interaction (FSI) are simulated by the standard MPM. On the other hand, the finite difference method (FDM) is employed to simulate the dispersion of the detonation products into the surrounding air where the particles degenerate to marker points which track the moving interface between detonation products and air. The conversion between MPM and FDM is implemented by the projection between the particles… More >

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