ROMINA J. BEVACQUA, RAFAEL FERNANDEZ-MARTIN, DANIEL F. SALAMONE
BIOCELL, Vol.35, No.1, pp. 1-7, 2011, DOI:10.32604/biocell.2011.35.001
Abstract Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human
embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos
inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the
second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to
block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the
diploid chromosome set. In experiment… More >
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