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  • Open Access


    Grapevine Phenological Quantitative Trait SSR Genotyping Using High-Throughput HRM-PCR Analysis

    Murad Awad1, Photini V. Mylona2, Alexios N. Polidoros1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 905-923, 2020, DOI:10.32604/phyton.2020.011235

    Abstract Discrimination among grapevine varieties based on quantitative traits, such as flowering, veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs. These traits are under complex genetic control for which 6 linked SSR loci (VVS2, VVIn16, VMC7G3, VrZAG29, VMC5G7, and VVIB23) have been identified. Using these markers in HRM-PCR analysis, we assessed genetic diversity among a large collection of 192 grapevine varieties. The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties, 3 prominent foreign varieties and 11 varieties of Palestinian origin. The SSR markers used were highly… More >

  • Open Access


    Association between microsatellites and resistance to Mal de Río Cuarto in maize by discriminant analysis

    Bonamico1 NC, MG Balzarini2, AT Arroyo2, MA Ibañez1, DG Díaz3, JC Salerno3, MA Di Renzo1

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 31-38, 2010, DOI:10.32604/phyton.2010.79.031

    Abstract Resistance to Mal de Río Cuarto (MRC) disease in maize (Zea mays L.) is important in Argentina because the crop area involves a wide region where the disease is endemic. Molecular marker-assisted selection could be used as an additional selection tool to enhance precision of the genotype selection for resistance. It demands the identification of informative markers. Microsatellite (SSR) markers linked to gene(s) associated with resistance to MRC have been reported from previous QTL analyses. These analyses have been made on linkage maps derived from a relatively early mapping population. In advanced populations, where highly distinct genotypes are easily classified,… More >

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