García-Zambrano2 EA, F Zavala-García2, A Gutiérrez-Diez, MC Ojeda-Zacarías, I Cerda-Hurtado3
Phyton-International Journal of Experimental Botany, Vol.78, pp. 117-120, 2009, DOI:10.32604/phyton.2009.78.117
Abstract The objective of this study was to apply the AFLP-type molecular marker technique to estimate genetic diversity in cactus pear within the Germplasm Bank of FAUANL. Twelve accessions have been reported as possible duplicates through type RAPD- molecular markers in such Bank. DNA was extracted using the cellular ruptor technique, and then digested and bound. A preamplification and subsequent selective amplification were conducted. Amplified fragments were then separated and analyzed. It was concluded that none of the accessions was duplicated. This was because 8 groups were formed in the dendogram obtained after applying the UPGMA method. There were 3 accessions… More >
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