García-Zambrano2 Eduardo A, Adriana Gutiérrez-Díez, Gilberto E Salinas-García, Elizabeth Cárdenas-Cerda, Rigoberto E Vázquez-Alvarado, Francisco Zavala-García, Jesús Martínez de la Cerda
Phyton-International Journal of Experimental Botany, Vol.75, pp. 125-135, 2006, DOI:10.32604/phyton.2006.75.125
Abstract The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 Opuntia accessions from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the Opuntia DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of… More >
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