DAOLUN YU¹, DEYONG SHE², KAI GE¹, LEI YANG¹, RUINA ZHAN¹, SHAN LU^3,*, YAFEI CAI^4,*

BIOCELL, Vol.48, No.1, pp. 139-147, 2024, DOI:10.32604/biocell.2023.045884 - 30 January 2024

Abstract Background: SMAD family proteins (SMADs) are crucial transcription factors downstream of transforming growth factor beta (TGF-ß)/SMAD signaling pathways that have been reported to play a pivotal role in mammalian reproduction. However, the role of SMAD family member 8 (SMAD8, also known as SMAD9), a member of the SMAD family, in mammalian reproduction remains unclear. Methods: We employed RNA interference techniques to knock down Smad8 expression in mouse granulosa cells (GCs) to investigate the effects of Smad8 on GC growth and steroidogenesis. Results: Our findings revealed a significant decrease in the proliferative capacity and a substantial increase in… More >

DANIELLA M. ADAMS¹, KALIE F. BECKERS¹, JULIET P. FLANAGAN¹, VIVIANE C. L. GOMES^1,#, CHIN-CHI LIU¹, JENNY L. SONES^1,2,*

BIOCELL, Vol.47, No.9, pp. 2051-2058, 2023, DOI:10.32604/biocell.2023.029644 - 28 September 2023

Abstract Background: Women with obesity have higher risk of adverse pregnancy outcomes, including preeclampsia (PE). Late-gestational hypertension, aberrant fetoplacental development, and fetal growth restriction (FGR), hallmarks of PE, are observed spontaneously in BPH/5 mice. Similar to obese preeclamptic women, BPH/5 mice have higher visceral white adipose tissue (WAT) and circulating leptin. We hypothesized that attenuation of maternal obesity and serum leptin in pregnant BPH/5 mice will improve fetoplacental development by decreasing hypoxia markers and leptin expression at the maternal-fetal interface. Methods: To test this hypothesis, BPH/5 mice were fed ad libitum (lib) and pair-fed (PF) to C57… More >

NAN TANG^1,#, YAJING ZHAN^1,#, JIAYAN MAO^2,#, ANKANG YIN¹, WEI WANG^3,*, JUAN WANG^3,*

BIOCELL, Vol.47, No.9, pp. 2009-2026, 2023, DOI:10.32604/biocell.2023.029269 - 28 September 2023

Abstract Non-small cell lung cancer (NSCLC) is a malignant tumor with high incidence worldwide. Triptolide (TP), extracted from Tripterygium wilfordii Hook F, exhibits potent broad-spectrum antitumor activity. Although some mechanisms through which TP inhibits NSCLC are well understood, those that involve ribosomal proteins remain yet to be understood. In this study, the transcriptome and proteome were integrated and analyzed. Our data indicated ribosomal protein L4 (RPL4) to be a core hub protein in the protein-protein interaction network. RPL4 is overexpressed in NSCLC tissues and cells. Transfection with siRPL4 or TP treatment alone arrested the cell cycle in More > Graphic Abstract

Shin Jin Kang¹, Soo Kyun Kim^2,*

Intelligent Automation & Soft Computing, Vol.35, No.3, pp. 2573-2588, 2023, DOI:10.32604/iasc.2023.029893 - 17 August 2022

Abstract In this paper, we propose an authentication method that use mouse and keystroke dynamics to enhance online privacy and security. The proposed method identifies personalized repeated user interface (UI) sequences by analyzing mouse and keyboard data. To this end, an Apriori algorithm based on the keystroke-level model (KLM) of the human–computer interface domain was used. The proposed system can detect repeated UI sequences based on KLM for authentication in the software. The effectiveness of the proposed method is verified through access testing using commercial applications that require intensive UI interactions. The results show using our More >

B. Kalpana¹, S. Dhanasekaran², T. Abirami³, Ashit Kumar Dutta⁴, Marwa Obayya⁵, Jaber S. Alzahrani⁶, Manar Ahmed Hamza^7,*

Computer Systems Science and Engineering, Vol.44, No.3, pp. 2243-2257, 2023, DOI:10.32604/csse.2023.027129 - 01 August 2022

Abstract Biomedical data classification has become a hot research topic in recent years, thanks to the latest technological advancements made in healthcare. Biomedical data is usually examined by physicians for decision making process in patient treatment. Since manual diagnosis is a tedious and time consuming task, numerous automated models, using Artificial Intelligence (AI) techniques, have been presented so far. With this motivation, the current research work presents a novel Biomedical Data Classification using Cat and Mouse Based Optimizer with AI (BDC-CMBOAI) technique. The aim of the proposed BDC-CMBOAI technique is to determine the occurrence of diseases… More >

XINFU FAN^1,2,#, JIE CHEN^3,#, ZONGMENG ZHANG^4,#, FANGHUI CHEN⁵, HAOSEN WANG⁶, YAFEI CAI⁶, JUN LI^7,*

BIOCELL, Vol.46, No.6, pp. 1527-1535, 2022, DOI:10.32604/biocell.2022.018269 - 07 February 2022

Abstract Spinal cord injury (SCI) remains an intractable clinical challenge of neurosurgery, it can be divided into two stages: uncontrollable primary injury induced by mechanical damage and controllable secondary injury regulated by continuous cell death. The apoptosis was the one of most important events in secondary injury, previous studies revealed that excessive endoplasmic reticulum (ER) stress breaks down the homeostasis and triggers apoptosis in the spinal cord. To deter or alleviate the secondary jury, we screen one of fat-soluble compounds, salubrinal, which was an inhibitor of eIF2α dephosphorylation can repair SCI by inhibiting ER stress in… More >

XINGYU MEI, ZHOUWEI WU, CHENGZHONG ZHANG, YUE SUN, WEIMIN SHI^*

BIOCELL, Vol.45, No.6, pp. 1551-1559, 2021, DOI:10.32604/biocell.2021.015376 - 01 September 2021

Abstract Vitiligo results in an autoimmune disorder destructing skin pigment cells, melanocytes (Mcs). This study aimed to investigate whether Astragaloside IV (AIV) could efficiently induce differentiation of bone marrow mesenchymal stem cells (BMMSCs) into Mcs. BMMSCs were induced and differentiated into Mcs with 0.1, 0.2, and 0.4 mg/L AIV during 150-day. Morphologic changes of differentiated cells were observed. Levels of some melanocytic specific genes (TRP-1, TRP-2, MART-1, Mitf) were measured with quantitative polymerase chain reaction (qPCR) at 90, 120, and 150 days of induction. After 90-day induction, the differentiated cells with 0.4 mg/L AIV demonstrated the typical morphology… More >

LINGHUI YU^#, RONG SHEN^#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG^*, DEGUI WANG^*

BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602 - 03 March 2021

Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of More >

Ming Jia^*1, Yulin Xiong^†1, Maoshi Li^*, Qing Mao^*

Oncology Research, Vol.28, No.4, pp. 371-383, 2020, DOI:10.3727/096504020X15853075736554

Abstract Chemotherapy is critical for the treatment of hepatocellular carcinoma (HCC). Despite the proapoptotic effects of corosolic acid (CA) treatment, its underlying mechanism is not completely clear. The aim of this study was to determine the molecular mechanism of CA in HCC treatment. MTT assay was used to determine the IC50 of CA. Immunoprecipitation and immunofluorescence were used to detect the interaction and subcellular localization of Yes-associated protein (YAP) and mouse double minute 2 (MDM2). In addition, in vivo xenotransplantation was performed to assess the effects of CA, YAP, and MDM2 on tumorigenesis. The IC50 of… More >

YI ZHENG^#, PENGFEI ZHANG^#, JINSHENG ZHU^#, LINGKAI ZHANG, WENXIAN ZENG^*

BIOCELL, Vol.44, No.2, pp. 201-207, 2020, DOI:10.32604/biocell.2020.08770 - 27 May 2020

Abstract Spermatogenesis is a highly efficient and intricate process in the testis by which mature spermatozoa are produced daily to maintain lifelong male fertility. Essential to this process are spermatogonia capable of both proliferation and differentiation. Nevertheless, the underlying mechanisms for spermatogonial proliferation and differentiation remain poorly understood. MicroRNAs (miRNAs) are a category of non-coding small RNAs with regulatory functions by binding to the 3’ untranslated region (UTR) of the target mRNA. Previous studies have demonstrated that miRNAs are capable of modulating cell proliferation, differentiation and apoptosis, but the roles of individual miRNAs in spermatogonial fate… More >