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  • Open Access

    ARTICLE

    Loss of the ubiquitin-conjugating enzyme Rad6B disturbs mitochondrial function and cellular homeostasis in mouse skin

    LINGHUI YU#, RONG SHEN#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG*, DEGUI WANG*

    BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602

    Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of Rad6B was characterized by an… More >

  • Open Access

    ARTICLE

    MicroRNA-382 inhibits the proliferation of mouse spermatogonia by targeting Kmt5a

    YI ZHENG#, PENGFEI ZHANG#, JINSHENG ZHU#, LINGKAI ZHANG, WENXIAN ZENG*

    BIOCELL, Vol.44, No.2, pp. 201-207, 2020, DOI:10.32604/biocell.2020.08770

    Abstract Spermatogenesis is a highly efficient and intricate process in the testis by which mature spermatozoa are produced daily to maintain lifelong male fertility. Essential to this process are spermatogonia capable of both proliferation and differentiation. Nevertheless, the underlying mechanisms for spermatogonial proliferation and differentiation remain poorly understood. MicroRNAs (miRNAs) are a category of non-coding small RNAs with regulatory functions by binding to the 3’ untranslated region (UTR) of the target mRNA. Previous studies have demonstrated that miRNAs are capable of modulating cell proliferation, differentiation and apoptosis, but the roles of individual miRNAs in spermatogonial fate determination remain largely elusive. Here,… More >

  • Open Access

    ARTICLE

    Brief Note: Isolation, culture, characterization and optimization of human corneal stem cells

    ALI M.SHARIFI1,2*, RADBOD DARABI2, AND KHOSROW JADIDI3

    BIOCELL, Vol.34, No.1, pp. 53-55, 2010, DOI:10.32604/biocell.2010.34.053

    Abstract The effects of human versus mouse EGF on cell growth and culture duration were studied to optimize a human limbal stem cells culture method for therapeutical autologous transplantation. Limbal cells were obtained by trypsin digestion and transferred to a culture medium. The time needed to reach full confluence in culture was determined. Specific antibodies to corneal stem cell marker (P63) versus corneal epithelial differentiation marker (K3) were used for histochemical determinations. A high proportion of P63 positive cells (85± 4.6%), and a correspondingly low proportion K3 positive cells (15 ± 3.8%) indicated that most cultured cells remained undifferentiated and were… More >

  • Open Access

    ARTICLE

    Sequential expressions of Notch1, Jagged2 and Math1 in molar tooth germ of mouse

    SILVIA S. BORKOSKY1,2, HITOSHI NAGATSUKA1, YORIHISA ORITA3, HIDETSUGU TSUJIGIWA4, JUNKO YOSHINOBU3, MEHMET GUNDUZ1, ANDREA P. RODRIGUEZ1, LILIANA R. MISSANA2, KAZUNORI NISHIZAKI3, NORIYUKI NAGAI1

    BIOCELL, Vol.32, No.3, pp. 251-257, 2008, DOI:10.32604/biocell.2008.32.251

    Abstract The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during… More >

  • Open Access

    ARTICLE

    Antioxidant capacity of vitamin C in mouse liver and kidney tissues

    ALICIA WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*

    BIOCELL, Vol.32, No.1, pp. 27-31, 2008, DOI:10.32604/biocell.2008.32.027

    Abstract In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice.
    The initial nmol of lipid hydroperoxides/g of tissue were 137 ± 11… More >

  • Open Access

    ARTICLE

    Regulation of mouse embryo development by autocrine throphic factors

    M.T. TERUEL1,2, R.C. CATALANO2, J.A. CABODEVILA2, S.S. CALLEJAS2

    BIOCELL, Vol.29, No.2, pp. 183-186, 2005, DOI:10.32604/biocell.2005.29.183

    Abstract Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos cultured for 24h… More >

  • Open Access

    ARTICLE

    Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver

    CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

    BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

    Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3… More >

  • Open Access

    ARTICLE

    Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development

    GRACIELA CRISTINA CALABRESE*, ROSA WAINSTOK**

    BIOCELL, Vol.28, No.3, pp. 251-258, 2004, DOI:10.32604/biocell.2004.28.251

    Abstract Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages.
    Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein… More >

  • Open Access

    ABSTRACT

    Fast Force Loading Disrupts Molecular Bond Stability in Human and Mouse Cell Adhesions

    Yunfeng Chen1,2,3,†,*, Jiexi Liao4,†, Zhou Yuan1, Kaitao Li4, Baoyu Liu4, Lining Arnold Ju4,5,6, Cheng Zhu1,2,4,5,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 97-97, 2019, DOI:10.32604/mcb.2019.07123

    Abstract Force-mediated molecular binding initiates numerous cellular activities such as cell adhesion, migration, and activation. Dynamic force spectroscopy (DFS) is widely used to examine molecular binding and cell mechano-signaling [1]. The rate of dissociation, off-rate, is an important attribute of molecular binding that reflects bond stability. Extensive DFS works have demonstrated that off-rates are a function of force magnitude, yielding signature bond behaviors like “catch bond” [2]. However, as a controversial topic of the field, different DFS assays, i.e., force-clamp and force-ramp assays, often yielded distinctive "off-rate vs. force" relations from the same molecular system [3]. Such discrepancies cast doubt on… More >

  • Open Access

    ABSTRACT

    Biomechanical Characterization of Mouse Sclera in Myopia

    C. Ross Ethier1,*, Dillon M. Brown1, Erica Landis2, Machelle T. Pardue1,2,3

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 61-63, 2019, DOI:10.32604/mcb.2019.07377

    Abstract Myopia, or near-sightedness, is a common ocular condition in which the eye elongates excessively. Development of myopia is associated with, and thought to be facilitated by, changes in the biomechanical properties of the sclera (the white part of the eye). We characterized scleral biomechanics in a mouse model of myopia using unconfirmed compression testing and biphasic theory to extract scleral permeability, in- plane scleral tensile modulus, and through-plane scleral compressive modulus. We find that myopia reduces in-plane tensile modulus and permeability, consistent with scleral tissue remodeling. Such biomechanical outcome measures may offer advantages over more traditional assessments of myopia-associated changes… More >

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