XINFU FAN^1,2,#, JIE CHEN^3,#, ZONGMENG ZHANG^4,#, FANGHUI CHEN⁵, HAOSEN WANG⁶, YAFEI CAI⁶, JUN LI^7,*

BIOCELL, Vol.46, No.6, pp. 1527-1535, 2022, DOI:10.32604/biocell.2022.018269

Abstract Spinal cord injury (SCI) remains an intractable clinical challenge of neurosurgery, it can be divided into two stages: uncontrollable primary injury induced by mechanical damage and controllable secondary injury regulated by continuous cell death. The apoptosis was the one of most important events in secondary injury, previous studies revealed that excessive endoplasmic reticulum (ER) stress breaks down the homeostasis and triggers apoptosis in the spinal cord. To deter or alleviate the secondary jury, we screen one of fat-soluble compounds, salubrinal, which was an inhibitor of eIF2α dephosphorylation can repair SCI by inhibiting ER stress in… More >

XINGYU MEI, ZHOUWEI WU, CHENGZHONG ZHANG, YUE SUN, WEIMIN SHI^*

BIOCELL, Vol.45, No.6, pp. 1551-1559, 2021, DOI:10.32604/biocell.2021.015376

Abstract Vitiligo results in an autoimmune disorder destructing skin pigment cells, melanocytes (Mcs). This study aimed to investigate whether Astragaloside IV (AIV) could efficiently induce differentiation of bone marrow mesenchymal stem cells (BMMSCs) into Mcs. BMMSCs were induced and differentiated into Mcs with 0.1, 0.2, and 0.4 mg/L AIV during 150-day. Morphologic changes of differentiated cells were observed. Levels of some melanocytic specific genes (TRP-1, TRP-2, MART-1, Mitf) were measured with quantitative polymerase chain reaction (qPCR) at 90, 120, and 150 days of induction. After 90-day induction, the differentiated cells with 0.4 mg/L AIV demonstrated the typical morphology… More >

LINGHUI YU^#, RONG SHEN^#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG^*, DEGUI WANG^*

BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602

Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of More >

YI ZHENG^#, PENGFEI ZHANG^#, JINSHENG ZHU^#, LINGKAI ZHANG, WENXIAN ZENG^*

BIOCELL, Vol.44, No.2, pp. 201-207, 2020, DOI:10.32604/biocell.2020.08770

Abstract Spermatogenesis is a highly efficient and intricate process in the testis by which mature spermatozoa are produced daily to maintain lifelong male fertility. Essential to this process are spermatogonia capable of both proliferation and differentiation. Nevertheless, the underlying mechanisms for spermatogonial proliferation and differentiation remain poorly understood. MicroRNAs (miRNAs) are a category of non-coding small RNAs with regulatory functions by binding to the 3’ untranslated region (UTR) of the target mRNA. Previous studies have demonstrated that miRNAs are capable of modulating cell proliferation, differentiation and apoptosis, but the roles of individual miRNAs in spermatogonial fate… More >

ALI M.SHARIFI^1,2*, RADBOD DARABI², AND KHOSROW JADIDI³

BIOCELL, Vol.34, No.1, pp. 53-55, 2010, DOI:10.32604/biocell.2010.34.053

Abstract The effects of human versus mouse EGF on cell growth and culture duration were studied to optimize a human limbal stem cells culture method for therapeutical autologous transplantation. Limbal cells were obtained by trypsin digestion and transferred to a culture medium. The time needed to reach full confluence in culture was determined. Specific antibodies to corneal stem cell marker (P63) versus corneal epithelial differentiation marker (K3) were used for histochemical determinations. A high proportion of P63 positive cells (85± 4.6%), and a correspondingly low proportion K3 positive cells (15 ± 3.8%) indicated that most cultured More >

SILVIA S. BORKOSKY^1,2, HITOSHI NAGATSUKA¹, YORIHISA ORITA³, HIDETSUGU TSUJIGIWA⁴, JUNKO YOSHINOBU³, MEHMET GUNDUZ¹, ANDREA P. RODRIGUEZ¹, LILIANA R. MISSANA², KAZUNORI NISHIZAKI³, NORIYUKI NAGAI¹

BIOCELL, Vol.32, No.3, pp. 251-257, 2008, DOI:10.32604/biocell.2008.32.251

Abstract The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15… More >

ALICIA WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*

BIOCELL, Vol.32, No.1, pp. 27-31, 2008, DOI:10.32604/biocell.2008.32.027

Abstract In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice.
The initial nmol of lipid hydroperoxides/g of tissue… More >

M.T. TERUEL^1,2, R.C. CATALANO², J.A. CABODEVILA², S.S. CALLEJAS²

BIOCELL, Vol.29, No.2, pp. 183-186, 2005, DOI:10.32604/biocell.2005.29.183

Abstract Embryo development depends on maternal and embryonic factors. When occurs in vitro, embryos secrete factors that stimulate their development. The purpose of this study was to investigate the possible effects of embryos at morula stage on mouse embryo development in vitro. To obtain conditioned media (CM), morulas were cultured in groups of 5 (CM5) or 10 (CM10) in microdrops of Ham-F10 culture medium during 24 h and later they were removed. Subsequently, 365 morulas were cultured in CM5 and CM10 or in Ham-F10 media (as control group). No differences in blastocyst formation could be found between embryos… More >

CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 More >

GRACIELA CRISTINA CALABRESE*, ROSA WAINSTOK**

BIOCELL, Vol.28, No.3, pp. 251-258, 2004, DOI:10.32604/biocell.2004.28.251

Abstract Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages.
Fluorescence activated cell sorter analysis showed a different… More >