Chiao-Ping Chen^1,2, Yan-Jei Tang^1,2, You-Yan Cai¹, Yi-Ru Pan³, Chun-Nan Yeh^3,4, Wen-Kuan Huang^1,2, Chih-Hong Lo^1,2, Yu-Tien Hsiao^1,2, Hsuan-Jen Shih^1,*, Chiao-En Wu^1,2,4,5,*

Oncology Research, Vol.33, No.11, pp. 3429-3446, 2025, DOI:10.32604/or.2025.066672 - 22 October 2025

Abstract Background: KIT proto-oncogene, receptor tyrosine kinase (KIT, CD117) and platelet-derived growth factor-alpha (PDGFRA) are key drivers of gastrointestinal stromal tumors (GIST), but resistance to targeted therapy often arises from tumor protein p53 (p53) alterations and loss of cell cycle control. However, the role of p53 status in GIST therapeutic potential has rarely been studied, so this study aimed to employ both wild-type and mutant p53 GIST models to investigate how p53 dysfunction influences the efficacy of p53 pathway-targeted therapies. Methods: The efficacy of the mouse double minute 2 homolog (MDM2) inhibitor (HDM201) and the Wee1… More >

Ya-Yi Chen^1,2, Tzu-Ting Chen³, Ya-Hsuan Chao⁴, Wen-Ho Chuo⁵, Chieh-Shan Wu^6,7, Ruo-Han Tseng^8,*, Chieh-Chen Huang^9,*

BIOCELL, Vol.49, No.9, pp. 1733-1748, 2025, DOI:10.32604/biocell.2025.067011 - 25 September 2025

Abstract Objectives: Professional antigen-presenting cells known as dendritic cells (DCs) assist as a connection between the innate and adaptive components of the immune response. DCs are attractive targets for immunomodulatory drugs because of their crucial function in triggering immunity. This study set out to examine, for the first time, how hibifolin affected mouse bone-marrow derived (BMDCs) dendritic cells, triggered by lipopolysaccharide (LPS) in vitro. Additionally, a mouse model of contact hypersensitivity (CHS) was used to assess its possible therapeutic effects in vivo. Methods: LPS was administered to BMDCs with or without hibifolin. Major Histocompatibility Complex (MHC) class II,… More >

Tatiana M. Astakhova, Nikita S. Karpov, Nataliya O. Dashenkova, Elena V. Alpeeva, Mikhail V. Nesterchuk, Sergey B. Akopov, Arsen S. Mikaelyan, Anfisa S. Ryabchenko, Pavel A. Erokhov, Natalia P. Sharova^*

Oncology Research, Vol.33, No.9, pp. 2573-2595, 2025, DOI:10.32604/or.2025.066611 - 28 August 2025

Abstract Objectives: Proteasomes, multi-subunit proteases, are key actors of cellular protein catabolism and a number of regulatory processes. The detection of subtle proteasome functioning in tumors may contribute to our understanding of the mechanisms of cancer development. The current study aimed to identify the role of low molecular mass protein 2 (LMP2), a proteasome immune subunit, in the development of mouse colon 26 (C26) adenocarcinoma. Methods: The functions of the LMP2 subunit in tumor development in Balb/c mice were studied using its irreversible inhibitor KZR-504. LMP2 activity was detected by the hydrolysis of the fluorogenic substrate… More >

Stanislav Aladev^1,*, Dmitry Sokolov¹, Maxim Politko¹, Galina Kazanskaya¹, Svetlana Aidagulova^1,2, Elvira Grigorieva^1,3

BIOCELL, Vol.49, No.6, pp. 1057-1069, 2025, DOI:10.32604/biocell.2025.064100 - 24 June 2025

Abstract Background: Glucocorticoids are used as anti-inflammatory drugs for the treatment of various diseases, however, their side effects on normal brain tissue remain underinvestigated. Objectives: The study aimed to investigate dexamethasone (DXM) effects on cell composition and myelin content in the mouse brain tissue. Methods: C57Bl/6 male mice (n = 60) received single and ten multiple intraperitoneal DXM injections (2.5 mg/kg), and the studied parameters were analysed at 1, 3, 7, 10 days after a single DXM injection and 15, 30, 60, and 90 days after the multiple injections. Oligodendrocytes, microglia, and astrocytes were assayed by immunohistochemistry… More >

ARTEM P. GUREEV¹, IRINA S. SADOVNIKOVA¹, EKATERINA V. CHERNYSHOVA¹, EKATERINA P. KRUTSKIKH¹, IRINA B. PEVZNER², LJUBAVA D. ZOROVA², VERONIKA V. NESTEROVA¹, POLINA I. BABENKOVA¹, EGOR Y. PLOTNIKOV^2,*

BIOCELL, Vol.49, No.5, pp. 873-892, 2025, DOI:10.32604/biocell.2025.063557 - 27 May 2025

Abstract Background: Mitochondrial dysfunction plays a critical role in the pathogenesis of Alzheimer’s disease (AD). Resveratrol is a promising compound for the treatment of various neurodegenerative diseases, including AD. Aims: To investigate mitochondrial damage and the effects of resveratrol on inflammation, cognitive function, and mitochondrial quality control in APP/PS1 mice. Methods: Comparative analysis of mitochondrial DNA (mtDNA) damage was conducted between 10-month-old APP/PS1 mice and age-matched C57BL/6 mice. Assessments included measurement of amyloid-β levels, inflammatory markers, swimming distance in the Morris water maze, and gut microbiome composition. Resveratrol’s effects on cytokine expression, mtDNA levels in plasma, and… More >

Alena A. Semenova^1,*, Anastasia D. Igoshkina¹, Alena A. Cherepanova¹, Natalia V. Mikina¹, Anastasia E. Stepanova¹, Оlga E. Krasnoshchekova¹, Vyacheslav A. Sharapov¹, Rimma G. Savchenko², Lyudmila V. Parfenova², Mikhail V. Dubinin¹

BIOCELL, Vol.49, No.3, pp. 437-450, 2025, DOI:10.32604/biocell.2025.061798 - 31 March 2025

Abstract Objectives: Skeletal muscle ischemia/reperfusion injury (IRI) occurs as a result of a marked reduction in arterial perfusion to a limb and can lead to tissue death and threaten limb viability. This work assessed the effects of 20-hydroxyecdysone (20E) on hindlimb skeletal tissue following tourniquet-induced ischemia/reperfusion injury. Methods: Animals were divided into 4 groups—control group (Control), Control + 20E (C + 20E), mice with IRI (IRI), and mice with IRI + 20E (IRI + 20E). IRI was modeled by applying a tourniquet to the hind limb for 2 h with reperfusion for 1 h. 5 mg/kg… More >

DONGYUN RONG^1,2,#, YUSHEN SU^1,#, ZHIRUI ZENG³, YAN YANG⁴, HONGUAN LU^1,*, YU CAO^1,*

Oncology Research, Vol.33, No.3, pp. 605-616, 2025, DOI:10.32604/or.2024.050661 - 28 February 2025

Abstract Background: Terpinen-4-ol (T4O), a key constituent of tea tree essential oil and various aromatic plants, has shown promising antiproliferative and pro-apoptotic effects in melanoma and other cancer types. However, its efficacy against cutaneous squamous cell carcinoma (cSCC) remains unclear. Thus, in this study, we investigated the in vivo and in vitro effects of T4O on cSCC cell lines and preliminarily explored its impacting pathways. Methods: Using CCK8 and assay colony formation, we assessed the viability of cSCC A431, SCL-1, and COLO-16 cells treated with T40 at varying concentrations (0, 1, 2, and 4 μM). Flow cytometry was… More >

ZULONG SHENG^*, YANRU HE, JUNYAN CAI, YUQIN JI, YUYU YAO, GENSHAN MA

BIOCELL, Vol.48, No.9, pp. 1369-1377, 2024, DOI:10.32604/biocell.2024.049905 - 04 September 2024

Abstract Background: Myocardial infarction (MI) is known worldwide for its important disabling features, including myocarditis and cardiomyocyte apoptosis. It is believed that microRNA (miRNA) has a role in the cellular processes of apoptosis and myocarditis, and miR-219a-5p has been found to suppress the inflammatory response. However, unknown is the precise mechanism by which miR-219a-5p contributes to MI. Methods: We measured the expression of miR-219a-5p and evaluated its effects on target proteins, inflammatory factors, and apoptosis in a mouse model of MI. Echocardiography was utilized to examine the MI clinical index, and triphenyl tetrazolium chloride staining was More >

Hung-Hsiang Wang¹, Chih-Ping Chen^2,*

CMES-Computer Modeling in Engineering & Sciences, Vol.140, No.1, pp. 685-709, 2024, DOI:10.32604/cmes.2024.047630 - 16 April 2024

Abstract This paper presents a new method of using a convolutional neural network (CNN) in machine learning to identify brand consistency by product appearance variation. In Experiment 1, we collected fifty mouse devices from the past thirty-five years from a renowned company to build a dataset consisting of product pictures with pre-defined design features of their appearance and functions. Results show that it is a challenge to distinguish periods for the subtle evolution of the mouse devices with such traditional methods as time series analysis and principal component analysis (PCA). In Experiment 2, we applied deep… More >

DANPING ZHU^1,#, GUANGMING LIU^1,#, KUAN FENG¹, SUYUN LI¹, DANDAN HU², SIDA YANG^3,*, PEIQING LI^1,*

BIOCELL, Vol.48, No.4, pp. 653-664, 2024, DOI:10.32604/biocell.2024.044856 - 09 April 2024

Abstract Background: During Enterovirus type 71 (EV71) infection, the structural viral protein 1 (VP1) activates endoplasmic reticulum (ER) stress associated with peripheral myelin protein 22 (PMP22) accumulation and induces autophagy. However, the specific mechanism behind this process remains elusive. Methods: In this research, we used the VP1-overexpressing mouse Schwann cells (SCs) models co-transfected with a PMP22 silencing or Autocrine motility factor receptor (AMFR/gp78) overexpressing vector to explore the regulation of gp78 on PMP22 and its relationship with autophagy and apoptosis. Results: The activity of gp78 could be influenced by EV71-VP1, leading to a decrease in the ubiquitination… More >