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  • Open Access


    Mouse cerebellar Purkinje cell damage induced by diphenylhydantoin acute intoxication

    Orlando J. CASTEJÓN*

    BIOCELL, Vol.39, No.2-3, pp. 33-40, 2015, DOI:10.32604/biocell.2015.39.033

    Abstract Twenty one days old Swiss albino mice that received diphenylhydantoin (25 mg/kg, i.p., daily for 15 days) progressively developed gait alterations, changes of behavior and cerebellar ataxia. Cerebellar slices were processed by conventional transmission electron microscopy. The body of Purkinje cells exhibited fragmented limiting plasma membranes, dilated nuclear envelopes, swelling and disassembly of nuclear pores, enlargement of rough and smooth endoplasmic reticulum and a notable detachment of membrane associated ribosomes, together with distorted vacuoles of smooth endoplasmic reticulum, bizarre shaped and swollen mitochondria with dilated cristae, as well as disrupted limiting lysosomal membranes. Degenerated axosomatic synapses apparently corresponding to basket… More >

  • Open Access


    Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model


    BIOCELL, Vol.26, No.1, pp. 35-48, 2002, DOI:10.32604/biocell.2002.26.035

    Abstract Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments were performed on groups… More >

  • Open Access


    Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation


    BIOCELL, Vol.31, No.2, pp. 237-245, 2007, DOI:10.32604/biocell.2007.31.237

    Abstract Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation… More >

  • Open Access


    Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species


    BIOCELL, Vol.31, No.1, pp. 51-59, 2007, DOI:10.32604/biocell.2007.31.051

    Abstract Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001).… More >

  • Open Access


    Gene expression profile of Sox1, Sox2, p53, Bax and Nestin in neural stem cells and adult mouse brain tissues


    BIOCELL, Vol.43, No.2, pp. 59-64, 2019, DOI:10.32604/biocell.2019.05731

    Abstract Histone deacetylation is a key modulator involved in cell proliferation, apoptosis, and mRNA transcription. However, the effects of histone deacetylation on C17.2 neural stem cells (NSCs) remain unclear. Here, the histone deacetylase inhibitors nicotinamide and trichostatin A (TSA) were used to determine the role of histone deacetylation on gene transcription in NSCs. The results showed that the mRNA expression of p53, Sox1, Sox2, and Bax were significantly higher in E14.5 NSCs than in C17.2 NSCs. Nestin, a marker gene of neuronal differentiation, did not differ significantly between E14.5 NSCs and C17.2 NSCs. The transcription levels of p53 and Nestin were… More >

  • Open Access


    Fast Force Loading Disrupts Molecular Binding Stability in Human and Mouse Cell Adhesions

    Yunfeng Chen1,2,3,†,*, Jiexi Liao4,†, Zhou Yuan1, Kaitao Li4, Baoyu Liu4, Lining Arnold Ju4,5,6, Cheng Zhu1,2,4,*

    Molecular & Cellular Biomechanics, Vol.16, No.3, pp. 211-223, 2019, DOI:10.32604/mcb.2019.07267

    Abstract Force plays critical roles in cell adhesion and mechano-signaling, partially by regulating the dissociation rate, i.e., off-rate, of receptor-ligand bonds. However, the mechanism of such regulation still remains elusive. As a controversial topic of the field, when measuring the “off-rate vs. force” relation of the same molecular system, different dynamic force spectroscopy (DFS) assays (namely, force-clamp and force-ramp assays) often yield contradictive results. Such discrepancies hurdled our further understanding of molecular binding, and casted doubt on the existing theoretical models. In this work, we used a live-cell DFS technique, biomembrane force probe, to measure the single-bond dissociation in three receptor-ligand… More >

  • Open Access


    Comparing the Effect of Uniaxial Cyclic Mechanical Stimulation and Chemical Factors on Myogenin and Myh2 Expression in Mouse Embryonic and Bone Marrow Derived Mesenchymal Stem Cells

    Norizadeh Abbariki Tannaz*,†, Shokrgozar Mohammad Ali†,‡, Haghighipour Nooshin*,§, Aghdami Nasser, Mahdian RezakII, Amanzadeh Amir*, Jazayeri Maryam*,†

    Molecular & Cellular Biomechanics, Vol.11, No.1, pp. 19-37, 2014, DOI:10.3970/mcb.2014.011.019

    Abstract Background: Environmental factors affect stem cell differentiation. In addition to chemical factors, mechanical signals have been suggested to enhance myogenic differentiation of stem cells. Therefore, this study was undertaken to illustrate and compare the effect of chemical and mechanical stimuli on Myogenin (MyoG) and Myosin heavy chani 2 (Myh2) expression of mouse bone marrowderived mesenchymal stem cells (BMSCs) and embryonic stem cells (ESCs). Methods: After isolation and expansion of BMSCs and generation of embryoid bodies and spontaneous differentiation of ESCs, cells were examined in 4 groups: (1) control group: untreated cells; (2) chemical group: cells incubated in myogenic medium (5-azacythidine… More >

  • Open Access


    Hypertrophic Gene Expression Induced by Chronic Stretch of Excised Mouse Heart Muscle

    Anna M. Raskin∗,†, Masahiko Hoshijima, Eric Swanson, Andrew D. McCulloch*, Jeffrey H. Omens∗,†,‡

    Molecular & Cellular Biomechanics, Vol.6, No.3, pp. 145-160, 2009, DOI:10.3970/mcb.2009.006.145

    Abstract Altered mechanical stress and strain in cardiac myocytes induce modifications in gene expression that affects cardiac remodeling and myocyte contractile function. To study the mechanisms of mechanotransduction in cardiomyocytes, probing alterations in mechanics and gene expression has been an effective strategy. However, previous studies are self-limited due to the general use of isolated neonatal rodent myocytes or intact animals. The main goal of this study was to develop a novel tissue culture chamber system for mouse myocardium that facilitates loading of cardiac tissue, while measuring tissue stress and deformation within a physiological environment. Intact mouse right ventricular papillary muscles were… More >

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