MIAOMIAO LI^1,#, ZILIN ZHENG^1,#, JUNYI KE¹, JIEYI LUO¹, FAN JIANG¹, YANXIA QU¹, BING ZHU², YINGHUA LI^2,*, LIANDONG ZUO^1,*

BIOCELL, Vol.47, No.6, pp. 1397-1405, 2023, DOI:10.32604/biocell.2023.027971

Abstract Background: Nano-selenium has been widely used in antiviral and anticancer therapy, and has the advantages of good targeting and low toxicity. For the first time, we combined male reproduction with nano-selenium to investigate its antioxidant effect. This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide (H₂O₂)-induced Leydig cell line, TM3. Methods: The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed. The concentration of 4 μM could significantly promote the growth of TM3 cells. Oxidative stress damage was caused using an 800 μM concentration of hydrogen peroxide.… More >

YONGLI WANG^1,2,#, SHENHONG QU^2,#, YONG YANG¹, YING QIN², FEI LIU³, GUANGWU HUANG^1,*

BIOCELL, Vol.47, No.3, pp. 533-545, 2023, DOI:10.32604/biocell.2023.025280

Abstract Background: Kinesin family member 15 (KIF15) is a protein that regulates cell mitosis and plays an important role in the development and progression of several types of human cancers. However, the role of KIF15 in the development of nasopharyngeal cancer (NPC) is still unclear. Methods: The differential expression of KIF15 in NPC and para-carcinoma tissues was evaluated based on data collected from Gene Expression Omnibus (GEO) database and immunohistochemical analysis of clinical specimens collected from a patient cohort. Cell lines 5-8F and CNE-2Z were selected for the construction of KIF15‑knockdown cell models. CCK8 assay, flow cytometry, wound healing, Transwell and… More >

Chunya Hu, Yu He^*

Oncologie, Vol.23, No.2, pp. 241-250, 2021, DOI:10.32604/Oncologie.2021.015828

Abstract Objective: The aim of the present study was to investigate the anti-tumor effects of formononetin on human nonsmall cell lung cancer (NSCLC) and its potential molecular mechanism. Methods: A549 cells were treated with different concentrations of formononetin, then detected the cell proliferation, apoptosis and the expression of HIPK2 respectively by MTT assay, flow cytometry analysis and RT-qPCR. Then the interaction between miR- 27a-3p and its target gene HIPK2 was verified through luciferase reporter assay. The expression of miR-27a- 3p, HIPK2, and p53 was detected after being treated with different formononetin concentrations by RT-qPCR and western blot. Results: The results showed… More >