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  • Open Access

    ARTICLE

    Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content

    Dandan Wang1,#, Yulin Guo1,#, Xiaofei Long1, Yan Pan1, Dingjie Yang1, Rui Li1, Ye Lu1, Ying Chen1,2, Jisen Shi1, Jinhui Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 27-34, 2020, DOI:10.32604/phyton.2020.08971 - 01 March 2020

    Abstract In order to study how exogenous hormones in C. lanceolata (gymnosperm) regulate somatic embryogenesis, we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis. By applying exogenous spermidine, we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content. Based on these results, further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic More >

  • Open Access

    ARTICLE

    Identification of phytohormone changes and its related genes under abiotic stresses in transgenic rice

    Mohammad UBAIDILLAH1,2, Miswar FAPERTA1,2, Kyung-Min KIM1

    BIOCELL, Vol.43, No.3, pp. 215-224, 2019, DOI:10.32604/biocell.2019.07549

    Abstract Abiotic stresses, such as drought and salinity, adversely affect plant growth and productivity. Comparison between non transgenic and transgenic rice harboring CaMsrB2 gene, which induces tolerance to abiotic stress, is important to observe response of gene under abiotic stress. Phytohormone showed a tendency to increase under the drought stress or salinity stress in the transgenic plant. RT-PCR analysis showed that gene expression and phytohormone levels under abiotic stress, to be closely related. The CaMsrB2 gene is related to the expression of JA and ABA hormones. Therefore, the level of expression of these genes and hormones was More >

  • Open Access

    ARTICLE

    Inhibitory effect of jasmonic acid and ethylene on epicotyl growth and bud induction in the maritime pine, Pinus pinaster Soland. in Ait

    MARIA TERESA MARTIN2*, HILDA PEDRANZANI3, PATRICIA GARCÍA-MOLINERO2, VALENTIN PANDO4, ROSARIO SIERRA-DE-GRADO1

    BIOCELL, Vol.33, No.3, pp. 141-148, 2009, DOI:10.32604/biocell.2009.33.141

    Abstract Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 μM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 μM. In addition to that, jasmonic acid… More >

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