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    Internal Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Potato Tissues

    Gang Li1, Yao Zhou2, Yaqi Zhao2, Yaxue Liu2, Yuwei Ke2, Xiaoqing Jin1, Haoli Ma1,2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 329-344, 2020, DOI:10.32604/phyton.2020.08874

    Abstract Quantitative real-time PCR (qRT-PCR) is widely used for investigating gene expression patterns and has many advantages, including its high sensitivity, fidelity, and specificity. Selecting a satisfactory internal reference gene is crucial for obtaining precise gene expression results in qRT-PCR analyses. In this study, the transcriptomic data of 2 potato varieties were screened for housekeeping genes with stable expression patterns. A total of 77 putative genes were selected, which were highly and stably expressed. Then, qRT-PCR analyses were performed to examine the expression levels of these 77 candidate reference genes in various potato tissues, including leaves, More >

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