Yinkai Xi1,2, Biao Zeng3, Hengyu Huang1,4,*
Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 987-1001, 2021, DOI:10.32604/phyton.2021.014218
Abstract An efficient regeneration system of Rhynchostylis retusa was established to provide technical reference for the
application of tissue culture tube seedlings in production. The mixtures of callus and protocorm from aseptic germination were used as explants. The optimal media of each stage was selected for callus proliferation, protocorm
occurrence and growth, rejuvenation and rooting via a single, complete combination and orthogonal experiment.
The results showed that the optimal medium for callus proliferation, protocorms occurrence and growth was 1/2
Murashige and Skoog (MS) medium adding 50 g·L−1 banana puree, 0.1 mg·L−1 α-naphthaleneacetic acid (NAA),
1.5 mg·L−1 6-benzylaminopurine (6-BA) and 1.0 mg·L−1… More >
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