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  • Open Access

    ARTICLE

    Label-free quantitative proteomics analysis models in vivo and in vitro reveal key proteins and potential roles in sciatic nerve injury

    YANG GU1,#,*, MINGGUANG BI2,#, DEHUI CHEN3, NING NI4, JIANMING CHEN1,*

    BIOCELL, Vol.47, No.9, pp. 2069-2080, 2023, DOI:10.32604/biocell.2023.029989

    Abstract Background: The underlying mechanism of sciatic nerve injury (SNI) is a common motor functional disorder, necessitates further research. Methods: A rat model of SNI was established, with the injury group subjected to compressive injury of the right sciatic nerve exposed at the midpoint of the thigh and the sham surgery group undergoing the same surgical procedure. An oxygen-glucose deprivation model was employed to simulate in vitro SNI in PC12 cells. Following data acquisition and quality control, differentially expressed proteins (DEPs) in each model were identified through differential analysis, and enrichment analysis was used to explore the potential functions and pathways… More >

  • Open Access

    ARTICLE

    Quantitative Proteomics Analysis Identifies the Potential Mechanism Underlying Yellow-Green Leave Mutant in Wheat

    Wei Zheng, Zheng Shi, Mei Long, Yuncheng Liao*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1147-1159, 2021, DOI:10.32604/phyton.2021.015916

    Abstract Enhancing photosynthesis efficiency is considered as one of the most crucial targets during wheat breeding. However, the molecular basis underlying high photosynthesis efficiency is not well understood up to now. In this study, we investigated the protein expression profile of wheat Jimai5265yg mutant, which is a yellow-green mutant with chlorophylls b deficiency but high photosynthesis efficiency. Though TMT-labeling quantitative proteomics analysis, a total of 72 differential expressed proteins (DEPs) were obtained between the mutant and wild type (WT). GO analysis found that they significantly enriched in thylakoid membrane, pigment binding, magnesium chelatase activity and response to light intensity. KEGG analysis… More >

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