Adel A. Rezk^1,2,*, Hala A. Amin²

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 691-705, 2023, DOI:10.32604/phyton.2022.024208

Abstract Citrus Tristeza Virus (CTV), usually occurs in nature as a mixture of genotypes. Six naturally infected citrus (Citrus sinensis) trees grafted on sour orange rootstock were collected from three citrus growing governorates in Egypt (Sharqia, Qalyubia and Garbia). In this study, RT-PCR, Single-Strand Conformation Polymorphism (SSCP) and nucleotide sequence analysis were used for four independent CTV genomic regions (p65, p18, p20, and p23) to detect and assess the sequence and genetic variabilities among CTV Egyptian isolates. RTPCR products (650 bp) for the CTV p23 gene obtained from the selected isolates were used for the SSCP analysis and DNA sequencing. SSCP… More >

Nan Pan^1*, Dilin Pan², Yi Liu², Gang Li³

Intelligent Automation & Soft Computing, Vol.25, No.2, pp. 367-373, 2019, DOI:10.31209/2019.100000098

Abstract A set of laser detection system for shearing tools is developed, By holding breakage of the cable, firstly, using single-point laser displacement sensors to pick up surface features signal of line trace, then wavelet decomposition is used to reduce the noise, and the signal after noise reduction is obtained. After that, the threshold based sequence comparison method is used to achieve matches of similar coincidence for trace features, and then using a gradient descent method to getting the minimum cost of cost function value through continuous iterative, and finally realizing the fast traceability of corresponding shearing tool. More >