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  • Open Access

    REVIEW

    Overview of Strain Characterization in Relation to Serological and Molecular Detection of Citrus tristeza Closterovirus

    Yasir Iftikhar1,*, Mazhar Abbas2, Mustansar Mubeen3, Muhammad Zafar-ul-Hye4,*, Faheema Bakhtawar1, Sonum Bashir1, Ashara Sajid1, Muhammad Asif Shabbir1

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1063-1074, 2021, DOI:10.32604/phyton.2021.015508

    Abstract Tristeza is a devastating viral disease in all the citrus growing countries throughout the world and has killed millions of citrus trees in severely affected orchards. The citrus species grafted on sour orange rootstock are affected by this disease. Predominantly, the sweet orange, grapefruit and lime trees grafted on sour orange exhibit severe symptoms like quick decline, vein clearing, pin holing, bark scaling and degeneration leading to variable symptoms. Symptomatic expression of Citrus tristeza virus (CTV) in different hosts has been attributed to virus isolates which are from severe to mild. Different serological and molecular assays have been deployed to… More >

  • Open Access

    ARTICLE

    Brief Note: Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I

    JOEL ARNEODO*, SOLEDAD DE BREUIL*, SERGIO LENARDON**, LUIS CONCI**

    BIOCELL, Vol.29, No.2, pp. 205-207, 2005, DOI:10.32604/biocell.2005.29.205

    Abstract Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina. More >

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