Xianrong Fu, Aiping Song, Bo Peng, Song Li, Weixin Liu, Lingling Zhang, Jiafu Jiang, Sumei Chen, Fadi Chen*
Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1681-1694, 2023, DOI:10.32604/phyton.2023.027870
Abstract Brassinosteroids (BRs), a class of steroid phytohormones, play a critical role in plant growth and development. The DWF4 gene encodes a cytochrome P450 enzyme (CYP90B1), which is considered a rate-limiting enzyme in BR biosynthesis. Here, we identified a homologous gene of DWF4 in chrysanthemum, CmDWF4. This gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point (pI) of 9.10. Overexpression of CmDWF4 in chrysanthemum was found to significantly increase growth rate, number, and length of lateral buds. Transcriptome analysis showed that multiple xyloglucan endotransglycosylase/hydrolase (XTH) family encoding genes associated with… More >
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