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  • Open Access


    Tissue Culture of Calla Lily (Zantedeschia spreng.): An Updated Review on the Present Scenario and Future Prospects

    Xuan Sun1,2, Xue Wang1, Bijaya Sharma Subedi3, Yin Jiang1,2, Di Wang1,2, Rongxin Gou1,2, Guojun Zhang2, Wenting Xu4,*, Zunzheng Wei1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2413-2428, 2023, DOI:10.32604/phyton.2023.029667

    Abstract The calla lily (Zantedeschia spreng.) is a bulbous flower native to the tropical regions of Africa. Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged flowering duration. Despite such advantages, for two sub-groups of calla lily, known as group Zantedeschia and group Aestivae, there are challenges in terms of hybrid production due to the ‘plastome-genome incompatibility’ therebetween. Tissue culture is a fundamental biotechnological tool used in gene editing research, with a focus on disease resistance and flower color in calla lily breeding programs. The present review provides a brief background on the… More >

  • Open Access


    Suspension Culture and Somatic Embryogenesis of Korean Pine

    Chunxue Peng1,2, Fang Gao1,2, Hao Wang1,2, Iraida Nikolaevna Tretyakova3, Alexander Mikhaylovich Nosov4,5, Hailong Shen1,2,*, Ling Yang1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 223-238, 2022, DOI:10.32604/phyton.2022.015523

    Abstract Korean pine is an important afforestation tree species in Northeast China, which has a high ecological and economic value. Although regeneration of somatic embryogenesis using immature zygotic embryos of Korean pine as explants has been successful, it cannot be applied to automation and large-scale production. Therefore, we urgently need a method that can increase the output of somatic embryos (SEs) to meet the needs of large-scale production. We used Korean pine 1–1 and 1–100 cell lines as research materials to evaluate the effects of inoculum-density, culture time, orbiting speed, vessel volume, plant growth regulator (PGR) concentration, and carbon source on… More >

  • Open Access


    The Regulatory Roles of microRNAs and Associated Target Genes during Early Somatic Embryogenesis in Liriodendron Sino-Americanum

    Dan Wang1, Fengjuan Lu1, Pengkai Wang1, Jiaji Zhang1, Lu Lu1, Yan Zhen1, Ye Lu1, Tielong Cheng2, Jisen Shi1,*, Jinhui Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1445-1463, 2021, DOI:10.32604/phyton.2021.015499

    Abstract Somatic cells respond to considerable stress, and go through a series of phytohormone pathways, then forming an embryo. The developmental process is recorded as somatic embryogenesis (SE). One of the key components regulating SE are the microRNAs (miRNAs). Despite previous studies, it is still not clear exactly how miRNAs exert their function of regulating targets during conditionally activated early SE. Here, we use Liriodendron sino-americanum as a model system and perform a combined analysis of microfluidic chips and degradome sequencing to study this process. We identified a total of 386 conserved miRNAs and 153 novel miRNAs during early SE. According… More >

  • Open Access


    Conservation Strategy for African Medicinal Species: In Vitro Biotechnological Approach

    Priyanka Jha1, Swati Chahal2,3, Devendra Kumar Pandey2,3, Joginder Singh4, Ram Prasad5,*, Vijay Kumar2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 779-794, 2020, DOI:10.32604/phyton.2020.09421

    Abstract The use of medicinal plants for different therapeutic values is well documented in African continent. African diverse biodiversity hotspots provide a wide range of endemic species, which ensures a potential medicinal value. The feasible conservation approach and sustainable harvesting for the medicinal species remains a huge challenge. However, conservation approach through different biotechnological tools such as micropropagation, somatic embryogenesis, synthetic seed production, hairy root culture, molecular markers based study and cryopreservation of endemic African medicinal species is much crucial. In this review, an attempt has been made to provide different in vitro biotechnological approaches for the conservation of African medicinal… More >

  • Open Access


    Exogenous Spermidine Promotes Somatic Embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content

    Dandan Wang1,#, Yulin Guo1,#, Xiaofei Long1, Yan Pan1, Dingjie Yang1, Rui Li1, Ye Lu1, Ying Chen1,2, Jisen Shi1, Jinhui Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 27-34, 2020, DOI:10.32604/phyton.2020.08971

    Abstract In order to study how exogenous hormones in C. lanceolata (gymnosperm) regulate somatic embryogenesis, we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis. By applying exogenous spermidine, we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content. Based on these results, further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis. Our research may… More >

  • Open Access


    Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

    Guan Z-J1,3,4*, S-B Lu2*,Y-L Huo5, B Guo3, Z-P Guan6, Y-H Wei3

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

    Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until… More >

  • Open Access


    Histocytological examination on organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

    Guan Z-J1,2,*, B Guo1,*, Y-L Huo3, J-K Dai4, Y-H Wei1

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 51-58, 2012, DOI:10.32604/phyton.2012.81.051

    Abstract The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron… More >

  • Open Access


    Stability of protein patterns in coffee seedlings regenerated by somatic embryogenesis

    Menéndez-Yuffá1 A, L Ríos-Bolívar2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

    Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a characteristic set of protein… More >

  • Open Access


    Somatic embryogenesis induction from immature embryos of Sorghum bicolor L. (Moench)

    Espinoza-Sánchez EA1, YA Sánchez-Peña2, JA Torres-Castillo3, EA García-Zambrano2, JE Treviño Ramírez2, F Zavala-García2, SR Sinagawa-García2

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105

    Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media composition and variety. The results… More >

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