Otilio Vázquez-Martínez1, Héctor Gordon Núñez-Palenius1,*, Eugenio M. Pérez-Molphe Balch2,*, Mauricio Valencia-Posadas1, Luis Pérez-Moreno1, Graciela M. L. Ruiz-Aguilar1, M. Gómez-Lim3
Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 83-96, 2022, DOI:10.32604/phyton.2022.017281
Abstract In Mexico, there is a need to produce large quantities of plantlets for the establishment and replanting of blue (cv. azul) agave production areas. Most of these plots are within the origin denomination area (DOT, Spanish acronym) of the distilled product of this plant, known as tequila. The objective of this study was to develop an in vitro-propagation protocol for Agave tequilana Weber cv. azul using segmented stems in both: solid and liquid media. A disinfection and in vitro technique were developed to obtain shoots, through plantlets collected in commercial plots, which attained 100% surface-disinfection and budding rate. At the… More >
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