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    ARTICLE

    Investigation of the Interaction Mechanism between Lignin Structural Units and Enzyme

    Lijing Huang, Penghui Li, Kangjie Jiang, Wenjuan Wu*

    Journal of Renewable Materials, Vol.11, No.4, pp. 1613-1626, 2023, DOI:10.32604/jrm.2022.023605

    Abstract The effect of lignin structural units on enzymatic hydrolysis of lignocellulosic biomass was investigated, especially the inhibitory role of lignin in non-productive adsorption with enzymes. Milled wood lignin (MWL) was isolated from different hardwoods of poplar, eucalyptus and acacia. The isolated lignin samples were characterized by elemental analysis, gel permeation chromatography, nitrobenzene oxidation and fourier infrared spectroscopy. The mechanism of lignin structural units on enzymatic hydrolysis of cellulose was studied by quartz crystal microbalance (QCM). The results showed that different structural units of lignin had different adsorption capacity for enzymes. The results of nitrobenzene oxidation indicated that the S/G ratio… More > Graphic Abstract

    Investigation of the Interaction Mechanism between Lignin Structural Units and Enzyme

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