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  • Open Access

    ARTICLE

    Classification of Human Protein in Multiple Cells Microscopy Images Using CNN

    Lina Al-joudi, Muhammad Arif*

    CMC-Computers, Materials & Continua, Vol.76, No.2, pp. 1763-1780, 2023, DOI:10.32604/cmc.2023.039413

    Abstract The subcellular localization of human proteins is vital for understanding the structure of human cells. Proteins play a significant role within human cells, as many different groups of proteins are located in a specific location to perform a particular function. Understanding these functions will help in discovering many diseases and developing their treatments. The importance of imaging analysis techniques, specifically in proteomics research, is becoming more prevalent. Despite recent advances in deep learning techniques for analyzing microscopy images, classification models have faced critical challenges in achieving high performance. Most protein subcellular images have a significant class imbalance. We use oversampling… More >

  • Open Access

    Analysis of Subcellular Localization and Pathogenicity of Plum Bark Necrosis Stem-Pitting Associated Virus Protein P6

    Yuanyuan Li1,#, Jinze Mu2,#, Qingliang Li1, Huabing Liu3, Xuefeng Yuan2,*, Deya Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2079-2085, 2023, DOI:10.32604/phyton.2023.028237

    Abstract Infection of plum bark necrosis stem pitting associated virus (PBNSPaV) has been reported in many Prunus species in several countries, causing significant economic losses. The very small proteins encoded by plant viruses are often overlooked due to their short sequences and uncertain significance. However, numerous studies have indicated that they might play important roles in the pathogenesis of virus infection. The role of small hydrophobic protein P6, encoded by the open reading frame 2 of PBNSPaV, has not been well explored. In this study, we amplified the P6 fragment from a PBNSPaV isolate by RT-PCR using specific primers and found… More >

  • Open Access

    ARTICLE

    Cloning and Characterization of EuGID1 in Eucommia ulmoides Oliver

    Yulu Chen1, Fen Zhang1, Ju Cai1, Yichen Zhao1,2, Jiaxue Cui1, Yan Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 999-1013, 2022, DOI:10.32604/phyton.2022.018984

    Abstract Gibberellic acid controlled the key developmental processes of the life cycle of landing plants, and regulated the growth and development of plants. In this study, a novel gibberellin receptor gene EuGID1 was obtained from Eucommia ulmoides Oliver. The cDNA of EuGID1 was 1556 bp, and the open reading frame was 1029 bp, which encoded 343 amino acids. EuGID1 had the homology sequence with the hormone-sensitive lipase family. Amino acid sequence alignment confirmed EuGID1 protein had the highest homology with the GID1 protein of Manihot esculenta. EuGID1 was located in the nucleus and cell membrane and had expression in four plant organs. Overexpression… More >

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