Fan Feng¹, Tingting Xia¹, Runze Zhao¹, Mengyue Wang¹, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 131-131, 2019, DOI:10.32604/mcb.2019.07211

Abstract Objective: To study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.
Results: With the aid of RNA-Seq technology, our study characterizes the transcriptome of hepatocytes cultured on soft, moderate, stiff and plastic substrates. Compared to soft substrate, our RNA-Seq results revealed 1131 genes that were up-regulated and 2534 that were down-regulated on moderate substrate, 1370 genes that were up-regulated and 2677 down-regulated genes on stiff substrate. Functional enrichment analysis indicated that differentially expressed genes were associated with the regulation of actin… More >

Jianyong Huang, Xiaoling Peng, Chunyang Xiong, Jing Fang

The International Conference on Computational & Experimental Engineering and Sciences, Vol.17, No.4, pp. 119-120, 2011, DOI:10.3970/icces.2011.017.119

Abstract Cell-substrate interfacial interplay plays a key role in many fundamental aspects of mammalian physiology. Recent progresses in related experiments concerning cell interactions with extracellular matrix have demonstrated that substrate rigidities can exert a remarkable influence on cell adhesion and spreading dynamics. For example, it has been reported that cell-substrate adhesion strength usually rises monotonically with Young's modulus of underlying elastic substrates and eventually achieves the strongest adhesion on rigid substrates such as glass, and that cell spreading area takes on a growing trend with the increase in substrate hardness and finally reaches a plateau when the stiffness exceeds the order… More >