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  • Open Access


    Gene Expression Profiling of Human Hepatocytes Grown on Differing Substrate Stiffness

    Fan Feng1, Tingting Xia1, Runze Zhao1, Mengyue Wang1, Li Yang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 131-131, 2019, DOI:10.32604/mcb.2019.07211

    Abstract Objective: To study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.
    Results: With the aid of RNA-Seq technology, our study characterizes the transcriptome of hepatocytes cultured on soft, moderate, stiff and plastic substrates. Compared to soft substrate, our RNA-Seq results revealed 1131 genes that were up-regulated and 2534 that were down-regulated on moderate substrate, 1370 genes that were up-regulated and 2677 down-regulated genes on stiff substrate. Functional enrichment analysis indicated that differentially expressed genes were associated with the regulation of actin… More >

  • Open Access


    Cell-substrate specific adhesion model regulated by substrate stiffness

    Jianyong Huang, Xiaoling Peng, Chunyang Xiong, Jing Fang

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.17, No.4, pp. 119-120, 2011, DOI:10.3970/icces.2011.017.119

    Abstract Cell-substrate interfacial interplay plays a key role in many fundamental aspects of mammalian physiology. Recent progresses in related experiments concerning cell interactions with extracellular matrix have demonstrated that substrate rigidities can exert a remarkable influence on cell adhesion and spreading dynamics. For example, it has been reported that cell-substrate adhesion strength usually rises monotonically with Young's modulus of underlying elastic substrates and eventually achieves the strongest adhesion on rigid substrates such as glass, and that cell spreading area takes on a growing trend with the increase in substrate hardness and finally reaches a plateau when the stiffness exceeds the order… More >

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