MIROSLAV VAŘECHA1*, DANIELA PÁCLOVÁ2, JIŘINA PROCHÁZKOVÁ2, PAVEL MATULA1, DUŠAN CMARKO3, AND MICHAL KOZUBEK1
BIOCELL, Vol.36, No.3, pp. 121-126, 2012, DOI:10.32604/biocell.2012.36.121
Abstract Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell… More >
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