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    ARTICLE

    OsMAPK6 Affects Male Fertility by Reducing Microspore Number and Delaying Tapetum Degradation in Oryza sativa L.

    Wuzhong Yin#, Hongxia Yang#, Yantong Wang, Ping Feng, Yao Deng, Yang Liu, Danyang Chen, Yijie Ban, Weichi Liu, Guanghua He*, Nan Wang*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 727-744, 2022, DOI:10.32604/phyton.2022.017811

    Abstract The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza sativa mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and transverse histological sectioning, and map-based cloning were performed to characterize the osmapk6 mutant. The gene OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the wild type,… More >

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