CHEN FENG*, CHEN XIANG-MING**, CHEN ZHI*, JIANG HAN-LIANG*, PAN XIAO-PING*, HU ZHONG-RONG*, LIU RONG-HUA*, CHEN XIAO-MING*
BIOCELL, Vol.29, No.3, pp. 253-259, 2005, DOI:10.32604/biocell.2005.29.253
Abstract We want to construct a yeast expression system for thymosin α1 (Tα1) to make the orally administered Tα1 preparation possible. The whole Tα1 DNA fragment was obtained by PCR. After being digested with restriction enzymes, it was cloned into pYES2 vector. Sequencing was performed to identify the recombinant. The sequence of Tα1 in recombinant coincided with the original one reported in Genbank. When pYES2-Tα1 plasmid was transformed into yeast, galactose instead of glucose was used to induce Tα1 expression. Western blot was performed to identify the quality of the expressed Tα1. Dried yeast containing pYEST2-Tα1 was fed to Balb/c mice… More >
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